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A Complete Culture System for Human Induced Pluripotent Stem Cells
Solutions for maintaining human induced pluripotent stem cell lines-feeder-free, defined conditions and single-cell enzymatic passaging provide unparalleled experimental flexibility
DEF-CS culture system as a complete solution to purity issues-promotes homogeneous undifferentiated cells with virtually no spontaneous differentiation
Description of the step-by-step protocol-includes passaging, guidelines for cell line transfer from other systems, and applications
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Make Your Own Hepatocytes from iPS Cells
Description of a standardized hepatocyte differentiation protocol-validated on 25 different hiPS cell lines, without the need for optimization
Development of panels of hiPSC-derived hepatocytes with diverse genetic backgrounds-ideal for drug metabolism and safety studies
Applications for disease modeling and safety toxicology-start with any patient-specific hiPS cell lines
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[2016 ¿Â¶óÀμ¼¹Ì³ª] Human iPS cellsÀÇ È¿°úÀûÀÎ È®´ë ¹è¾çÀ» À§ÇÑ Cellartis¢ç DEF-CS Culture System
- Human induced pluripotent stem cell lines À¯Áö ¹è¾ç
feeder-free, defined conditionÀ¸·Î single-cell enzymatic passaging °¡´É
- ¹ÌºÐÈ»óÅ À¯Áö¿¡ Ź¿ùÇÑ DEF-CS ¹è¾ç ½Ã½ºÅÛ
ºñÀǵµÀû ºÐȾøÀÌ µ¿ÁúÀÇ ¹ÌºÐÈ ¼¼Æ÷ »óÅ À¯Áö
- Genome editing ÈÄ ¶Ù¾î³ single cell cloning È¿À²
hiPS cell colonyÀÇ °·ÂÇÑ proliferation°ú single cell cloning ÈÄ pluripotency À¯Áö
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[2016 ¿Â¶óÀμ¼¹Ì³ª] Human iPS cell À¯·¡ Hepatocyte - µ¶¼º ¿¬±¸¸¦ À§ÇÑ Çõ½ÅÀûÀÎ Tool
±âÁ¸ µ¶¼º Å×½ºÆ®¿¡ ÀÌ¿ëµÇ´Â primary hepatocytes, hepatoma cell line, animal model µî°ú °°Àº ½ÇÇè ¸ðµ¨¿¡ ´ëÇÑ ´ë¾ÈÀ¸·Î ¶°¿À¸£´Â human iPS cell À¯·¡ÀÇ hepatocyte¿¡ °üÇÏ¿© Ç¥ÁØ ÇÁ·ÎÅäÄÝÀ» ÀÌ¿ëÇÑ ºÐÈ °úÁ¤°ú Ư¼º °ËÁõ ¹× Àû¿ë ¿¹¸¦ ¼Ò°³ÇÏ°íÀÚ ÇÑ´Ù.
* Ç¥ÁØÈÇÑ hepatocyte ºÐÈ ÇÁ·ÎÅäÄÝ ¼³¸í
- 25°³ÀÇ hiPS cell lines¿¡¼ º°µµÀÇ ÃÖÀûÈ °úÁ¤ ¾øÀÌ ºÐÈ °ËÁõ
* ´Ù¾çÇÑ genetic background¸¦ °¡Áø hiPSC-derived hepatocyte ÆгÎ
- ¾à¹° ´ë»ç ¿¬±¸³ª ¾ÈÀü¼º ¿¬±¸¿¡ ÀÌ»óÀû
* Áúº´ ¸ðµ¨À̳ª µ¶¼º Å×½ºÆ® Àû¿ë¿¡ À¯¿ë
- ȯÀÚ Æ¯ÀÌÀû hiPS cell linesÀ¸·Î ½ÃÀÛÇϰųª ±âÁ¸ ¸¸µé¾îÁø hiPS-HEP cells·Î Àû¿ë °¡´É
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[2017 ¿Â¶óÀμ¼¹Ì³ª] ½Å¾à°³¹ßÀ» À§ÇÑ °³¼±µÈ Àΰ£ °£¼¼Æ÷ ¸ðµ¨
º» ¼¼¹Ì³ª¿¡¼´Â ±âÁ¸ÀÇ primary hepatocyte model¿¡¼ ºÒ°¡´É Çß´ø Hepatitis virus infection, chronic toxicity study¸¦ À§ÇØ
4ÁÖ°£ Àå±â¹è¾çÀÌ °¡´ÉÇÑ human primary hepatocyte medium. ´ëÇü assay ¹× ½Å¾à screeningÀ» À§ÇØ ´ë·®»ý»êÀÌ °¡´ÉÇÑ human iPS derived hepatocyteµî
È¿À²ÀûÀÎ ½Å¾à°³¹ßÀ» À§ÇÑ °³¼±µÈ Àΰ£ °£¼¼Æ÷ ¸ðµ¨À» ¼Ò°³ÇÕ´Ï´Ù
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[2017 ¿Â¶óÀμ¼¹Ì³ª] 2017³â »ýÈÇÐ ºÐÀÚ»ý¹°ÇÐȸ ¾ÞÄÝ ¼¼¹Ì³ª: Gene Editing of Human iPS cells
ÇÐȸ¿¡ Âü¼®ÇÏÁö ¸øÇϽŠ°í°´ ºÐµéÀÇ ¸¹Àº °ü½É¿¡ µû¶ó µ¿Àϳ»¿ëÀ¸·Î ÁøÇàµÈ Çѱ¹¾î ¹öÀü ¾ÞÄÝ ¼¼¹Ì³ª ÀÔ´Ï´Ù.
CRISPR/Cas9À» »ç¿ëÇÑ human iPS cell À¯ÀüÀÚ ±³Á¤°ú DEF-CS¢â 500 culture systemÀ» »ç¿ëÇÑ human iPS cell clonal line Á¦ÀÛ°úÁ¤À» ¼Ò°³ÇÕ´Ï´Ù
- Guide-it¢â Recombinant Cas9
Cas9-sgRNA RNP complex »ç¿ëÀ¸·Î gene editingÀÇ ¾ÈÁ¤¼º°ú ƯÀ̼º Çâ»ó
- Cellartis¢ç DEF-CS¢â 500
Monolayer culture·Î °íÈ¿À²ÀÇ gene editing °¡´É
°·ÂÇÑ human iPS single cell cloning ´É·Â
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