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Á¦ÇÑÈ¿¼Ò ¡¤ Quickcut
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Á¦ÇÑÈ¿¼Ò (C, D)
> Dpn I
Dpn I
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Takara
1235A
Dpn I
1000 U
76,000¿ø
Takara
1235B
Dpn I
5000 U (1235A x 5)
345,000¿ø
Product index
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16 ½Ã°£ ¹ÝÀÀ ½Ã ¿ÏÀüºÐ..
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Á¦ÇÑÈ¿¼Ò Çü»ó À϶÷
Á¦ÇÑÈ¿¼Ò È°¼º¿¡ ´ëÇÑ M..
Á¦ÇÑÈ¿¼Ò Genome DNA An..
Á¦ÇÑÈ¿¼ÒÀÇ »ç¿ë¿¡ °üÇÏ..
Á¦ÇÑÈ¿¼ÒÀÇ Star È°¼º
Basal Buffer Á¶¼º
Basal BufferÀÇ ¿°³óµµ..
Double Digestion¿ë Ãß..
Ligation-Recutting È¿..
pBR322¡¢pUC19ÀÇ ¿ÏÀüºÐ..
pKF3 Cloning Test
pKF3ÀÇ ¿ÏÀüºÐÇØ¿¡ ÇÊ¿ä..
Universal Buffer ¡¤ Ba..
Á¦ÇÑÈ¿¼Ò (A)
Aat II
Acc I
Acc II £¨FnuD II£©
Acc III £¨BspM II£©
Afa I £¨Rsa I£©
Afl II
Alu I
Aor13H I £¨BspM II, Ac..
Aor51H I £¨Eco47 III£©
Apa I
ApaL I
Á¦ÇÑÈ¿¼Ò (B)
Bal I
BamH I
Ban II £¨HgiJ II£©
BciT130 I
Bcn I £¨Cau II£©
Bgl I
Bgl II
Bln I £¨Avr II£©
BmeT110 I £¨Ava I£©
Bpu1102 I £¨Esp I£©
Bsp1286 I £¨Sdu I£©
Bsp1407 I
BspT104 I £¨Asu II, Ns..
BspT107 I £¨HgiC I£©
BssH II £¨BseP I£©
BstP I £¨BstE II, EcoO..
BstX I
Á¦ÇÑÈ¿¼Ò (C, D)
Cfr10 I
Cla I
Cpo I £¨Rsr II£©
Dde I
Dpn I
Dra I £¨Aha III£©
Á¦ÇÑÈ¿¼Ò (E, F)
Eae I £¨Cfr I£©
Eco52 I £¨Xma III£©
Eco81 I £¨Sau I£©
EcoO109 I £¨Dra II£©
EcoO65 I £¨BstE II, Bs..
EcoR I
EcoR V
EcoT14 I £¨Sty I£©
EcoT22 I £¨Ava III£©
Fba I £¨Bcl I£©
Fok I
Á¦ÇÑÈ¿¼Ò (H, K)
Hae II
Hae III
Hap II £¨Hpa II, Msp I..
Hha I
Hinc II £¨Hind II£©
Hind III
Hinf I
Hpa I
Kpn I
Á¦ÇÑÈ¿¼Ò (M, N)
Mbo I £¨Sau3A I£©
Mbo II
Mfl I £¨Xho II£©
Mlu I
Msp I £¨Hap II, Hpa II..
Mun I £¨Mfe I£©
Nae I
Nco I
Nde I
Nhe I
Not I
Nru I
Á¦ÇÑÈ¿¼Ò (P, R)
PmaC I
Psp1406 I £¨Acl I£©
Pst I
Pvu I
Pvu II
RspRS II (Mse I)
Á¦ÇÑÈ¿¼Ò (S, T)
Sac I
Sac II
Sal I
Sau3A I £¨Mbo I£©
Sca I
Sfi I
Sma I
Smi I £¨Swa I£©
SnaB I
Spe I
Sph I
Sse8387 I
Ssp I
Stu I
Taq I £¨TthHB8 I£©
Á¦ÇÑÈ¿¼Ò (V, X)
Van91 I (PflM I)
VpaK11B I £¨Ava II£©
Xba I
Xho I
Xsp I£¨Bfa I, Mae I£©
RNAÁ¦ÇÑÈ¿¼Ò (mRNA Interfer..
mRNA Interferase¢â -Ma..
Á¦ÇÑÈ¿¼Ò QuickCut
QuickCut¢â Restriction..
QuickCut (A)
QuickCut¢â Afa I (Rsa ..
QuickCut¢â Afl II
QuickCut¢â Alu I
QuickCut¢â Apa I
QuickCut (B)
QuickCut¢â BamH I
QuickCut¢â Bgl II
QuickCut¢â Bln I (Avr ..
QuickCut (C)
QuickCut¢â Cla I
QuickCut (D)
QuickCut¢â Dpn I
QuickCut¢â Dra I (Aha ..
QuickCut (E)
QuickCut¢â EcoR I
QuickCut¢â EcoR V
QuickCut (H)
QuickCut¢â Hae III
QuickCut¢â Hha I
QuickCut¢â Hind III
QuickCut¢â Hinf I
QuickCut¢â Hpa I
QuickCut (K)
QuickCut¢â Kpn I
QuickCut (M)
QuickCut¢â Mlu I
QuickCut (N)
QuickCut¢â Nco I
QuickCut¢â Nde I
QuickCut¢â Nhe I
QuickCut¢â Not I
QuickCut (P)
QuickCut¢â Pst I
QuickCut¢â Pvu I
QuickCut¢â Pvu II
QuickCut (S)
QuickCut¢â Sac I
QuickCut¢â Sac II
QuickCut¢â Sal I
QuickCut¢â Sfi I
QuickCut¢â Sma I
QuickCut¢â SnaB I
QuickCut¢â Spe I
QuickCut¢â Sph I
QuickCut¢â Stu I
QuickCut (X)
QuickCut¢â Xba I
QuickCut¢â Xho I
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10 U/§¡
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T buffer
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Universal Buffer ¡¤ Basal Buffer¿¡ ÀÇÇÑ Á¦ÇÑÈ¿¼Ò È°¼º Ç¥½Ã ½Ã½ºÅÛ
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pBR322 DNA (dam
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Escherichia coli
carrying the plasmid encoding
Dpn
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ATC (¸ÞÆ¿È µÈ A ¿°±â)´Â Àý´ÜÇÏÁö¸¸, GATC (¸ÞÆ¿È°¡ µÇÁö ¾ÊÀº A ¿°±â)´Â Àý´ÜÇÏÁö ¾Ê´Â´Ù.
ÀϹÝÀûÀÎ ´ëÀå±Õ (dam
+
±ÕÁÖ)·ÎºÎÅÍ ÇÕ¼ºµÈ DNA´Â Àý´ÜÇÏÁö¸¸ PCR »ê¹°Àº Àý´ÜÇÏÁö ¾Ê´Â´Ù.
Dam methylaseÀÇ ¿µÇâÀ» ¹ÞÁö ¾Ê´Â´Ù.
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Long fragment ÁõÆø ¹× GC-rich template ÁõÆø¿¡ °ÇÑ High Fidelity PCR È¿¼Ò:
PrimeSTAR
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GXL DNA Polymerase
°íÈ¿À²ÀÇ Ligation premix:
DNA Ligation Kit £¼Mighty Mix£¾
TA-cloningÀ» À§ÇÑ °íÈ¿À²ÀÇ Ligation premix ¿Í T-vector:
Mighty TA-cloning Kit
PCR°ú ´Ù¸¥ È¿¼Òó¸® ¹ÝÀÀ ÈÄ DNA fragment¸¦ Á¤Á¦Çϱâ À§ÇÑ:
TaKaRa MiniBEST DNA Fragment Purification Kit
Keyword :
Á¦ÇÑÈ¿¼Ò (C, D)
Cfr10 I
Cla I
Cpo I £¨Rsr II£©
Dde I
Dpn I
Dra I £¨Aha III£©