|
PrimeScript RTase (200 U/㎕)
|
10 ㎕
|
|
RNase Inhibitor (40 U/㎕)
|
10 ㎕
|
|
Oligo (dT)18 Primer (1 ㎍/㎕)
|
20 ㎕
|
|
Random Primer(9 mer) (0.3 ㎍/㎕)
|
20 ㎕
|
|
5X 1st Strand Synthesis Buffer
|
40 ㎕
|
|
dNTP Mixture (각 10 mM)
|
40 ㎕
|
|
E. coli RNase H / E. coli DNA Ligase Mixture
|
20 ㎕
|
|
E. coli DNA Polymerase I (20 U/㎕)
|
20 ㎕
|
|
5X 2nd Strand Synthesis Buffer
|
300 ㎕
|
|
T4 DNA Polymerase (1 U/㎕)
|
40 ㎕
|
|
RNase free dH2O
|
600 ㎕×2
|
|
Control RNA (1 ㎍/㎕)*
|
5 ㎕
|
