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High Input Strand- Specific Total RNA-Seq

High Input Stranded RNA-Seq Kit

제조사	제품코드	제품명	용량	가격 (부가세별도)	비고
Clontech	634873	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	12 회 (Package)	2,294,000원	재고 소진 후 종매
Clontech	634874	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	24 회 (Package)	3,872,000원	재고 소진 후 종매
Clontech	634875	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	48 회 (Package)	6,824,000원	재고 소진 후 종매
Clontech	634876	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	96 회 (Package)	9,288,000원	재고 소진 후 종매
Clontech	634877	SMARTer® Stranded Total RNA Sample Prep Kit - HI Mammalian	192 회 (Package)	17,882,000원	재고 소진 후 종매

Product index

PGT-A
- Embgenix™ PGT-A Kit (..
SMARTer RNA-Seq 개요
mRNA-Seq (Single cell)
Stranded RNA-seq
Small RNA-Seq
- Small RNA-Seq Kit
Immune-Seq
기타 RNA-Seq
- Full length cDNA 합성
- Target RNA Capture for..
DNA-seq 개요
- [기술개요] ThruPLEX & ..
- DNA-Seq 선택가이드
ChIP-Seq / Meth-Seq
Low Input DNA-Seq
High input DNA-seq
- ThruPLEX® DNA-Seq HV
- ThruPLEX® Tag-Seq HV
Single cell DNA-Seq
- PicoPLEX® Single Cell..
Whole Genome Amplification..
Index Kit
NGS Library 정량
- Library Quantification..
NGS Sample preparation
NGS 분석용 PCR 효소
NGS 관련 장비
[Legacy] NGS Products
- (Legacy) PicoPLEX® WG..

※ 안내
본 제품은 SMART-Seq Total RNA High Input (RiboGone Mammalian) (Code 635045, 635046, 635047) 제품으로 리뉴얼되었습니다.
자세한 내용은 다카라코리아 (02-2081-2510)로 문의 주세요.

SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian은 100ng - 1ug의 mammalian Total RNA로부터 Illumina용 sequencing library를 빠른 시간 내에 제작할 수 있다. 본 제품은 Total RNA의 90%를 이루는 ribosomal RNA (rRNA)를 제거를 위한 RiboGone (Code 634846)과 SMARTer 기술이 적용되어 있어 Total RNA로부터 약 5시간만에 rRNA depletion과 strand-specific RNA-Seq library를 제작할 수 있다.
SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian은 일반적인 양 (High-input, >100 ng)을 가진 RNA로부터 5시간만에 Illumina specific, indexed RNA Seq library를 제작하는 제품으로, 12개의 Reverse primer와 8개의 Forward primer를 포함하고 있어 총 96개의 indexed library를 제작 가능하다.

미량의 샘플(Low input, 100 pg- 100 ng)의 total RNA 샘플을 이용하는 경우, SMARTer Stranded Total RNA Sample Prep Kit - Low Input Mammalian (Code 634861)을 권장한다.

특징

Strand information - Identify each transcript’s strand of origin with >99% accuracy.
Fast, streamlined protocol - Go from start to finish in ~5 hours.
Large input size - Use 100 ng-1 μg total RNA from human, mouse, or rat.
Versatility - Get reliable data across replicates and RNA quality. Seehigh - quality, reproducible data from total RNA with a RIN (RNA IntegrityNumber) from 3-10.
Integrated with Illuminasequencing - Generate Illumina - specificlibraries with up to 96 indexes, and retain strand information.

그림 1. Flowchart of SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian library generation. Section A. Depletion of rRNA from total RNA samples with RiboGone technology. Section B. First-strand cDNA synthesis with SMART technology, incorporating Illumina Read Primers 1 and 2. Section C. Template switching and generation of sequencing libraries with Illumina cluster-generating sequences and indexes by PCR amplification.

표 1. High-quality libraries across varying levels of RNA quality. Libraries were generated from Mouse Liver RNA by chemically shearing until it had a RIN of 3 or 7. Sequencing data showed the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

표 2. Sequence Alignment Metrics. 400 ng of HURR and HBRR with ERCC Spike-In RNA were treated with this kit. Alignment data is displayed for both libraries, with the percentage of reads that mapped to rRNA, exonic regions, intronic regions, intergenic regions, and the correct strand, as defined by Picard analysis.

적용

Illumina-specific NGSlibrary generation that retains strand information
RNA-Seq on Illuminaplatforms for mammalian samples
Coding and non-codingtranscript information

구성품 (자세한 구성품은 CoA 또는 매뉴얼 참조)

- SMARTer Stranded Total RNA Sample Prep Kit - HI Mammalian Components
- Indexing Primer Set HT for Illumina - 12
- SeqAmp DNA Polymerase

Keyword :

Stranded RNA-seq

(with UMI) FFPE, LCM, cell-free RNA등의 극소..

[New](+UMI) Pico input stranded RNA-Seq Kit

FFPE, LCM, cell-free RNA등의 극소량 low qual..

[New]Pico input stranded RNA-Seq Kit

단일세포 (Single cell)에서 stranded RNA-Seq ..

[New]Single cell stranded RNA-Seq Kit

Mid Input Strand- Specific Total RNA-Seq

[New]Mid Input Stranded RNA-Seq Kit

High Input Strand- Specific Total RNA-Seq

[New]High Input Stranded RNA-Seq Kit

Total RNA-Seq Kit (FFPE, LCM)

UDI, UMI 적용으로 더욱 특별해진

임상샘플 최적 Pico Input Mammalian(v3)

Picogram RNA로부터 Strand-specific illumina ..

임상샘플 최적 Pico Input Mammalian(v2)

단일세포 (single cell)의 stranded RNA-Seq li..

Single cell Stranded RNA-Seq Kit

Mid Input Stranded RNA-Seq Kit

High Input Strand- Specific Total RNA-Seq

High Input Stranded RNA-Seq Kit