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Home > ÀüÁ¦Ç°º¸±â > Ç×ü ¡¤ ELISA ¡¤ Signal > EIA Kit > Rat Heme Oxygenase-1 EIA Kit
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Rat Heme Oxygenase-1 EIA Kit

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1. Anti-rat HO-1 Monoclonal Antibody Plate 96 well (8 well¡¿12 strips)¡¿1¸Å
2. PeroxidaseÇ¥½Ä HO-1 Antibody (µ¿°á°ÇÁ¶Ç°) 11 §¢ ¿ë
3. Ç¥ÁØÇ° (rat HO-1:µ¿°á°ÇÁ¶Ç°) 5 §¢ ¿ë
4. °Ëü Èñ¼®¾× 11 §¢ ¡¿ 2
5. ±âÁú¾× (TMBZ; 3,3¢¥5,5¢¥-tetramethylbenzidine) 12 §¢
6. ¹ÝÀÀÁ¤Áö¾× (1N Ȳ»ê) 12 §¢
7. Cell Extraction Buffer 11 §¢
º¸Á¸ 2~8¡É
Á¦Ç°¼³¸í
º» Å°Æ®Àº 2Á¾ÀÇ anti-rat heme oxygenase 1 monoclonal antibody (GTS-1, GTS-3)¸¦ Á¶ÇÕÇÏ¿© ±¸ÃàÇÑ sandwich typeÀÇ ELISA Å°Æ®ÀÌ´Ù. Rat Ç÷ûÀ̳ª Á¶Á÷ ¶Ç´Â rat ¹è¾ç ¼¼Æ÷³ª ¹è¾ç»óÃþ¿¡ ³ªÅ¸³­ rat heme oxygenase 1À» °£ÆíÇÏ°Ô ÃøÁ¤ÇÒ ¼ö ÀÖÀ¸¸ç (»ç¶÷ÀÇ Ç׿øÀº ÃøÁ¤ÇÒ ¼ö ¾øÀ½) Á¶ÀÛ ½Ã°£Àº 2½Ã°£ 30ºÐÀÌ´Ù.
HemoglobinÀ» ºñ·ÔÇÑ heme ´Ü¹éÁúÀÇ º¸°á ºÐÀÚÁ·ÀÎ hemeÀ» ´ãÁó»ö¼Ò(bilibverdin, bilirubin)¿Í ÀÏ»êȭź¼Ò (CO) ±×¸®°í ȯ¿øö (Fe2+)·Î ºÐÇØÇÏ´Â È¿¼ÒÀÌ´Ù. Hemeoxygenase´Â ÃÖ¼Ò µÎ °¡ÁöÀÇ isozyme (Heme Oxygenase-1, Heme Oxygenase-2)ÀÌ º¸°íµÇ°í ÀÖ´Ù. Heme Oxygenase-2´Â ±¸¼ºÇü È¿¼ÒÀÌ°í, Heme Oxygenase 1 (HO-1)Àº °¢Á¾ ½ºÆ®·¹½º¿ø (Á߱ݼÓ, endotoxin, Àڿܼ±, heat shock, È°¼º »ê¼Ò, Àú»ê¼Ò »óÅÂ)¿¡ ¹ÝÀÀÇÏ¿© ¼¼Æ÷³»¿¡¼­ À¯µµ ¹ßÇöµÇ´Â È¿¼ÒÀÌ´Ù. Heme Oxygenase¿¡ ÀÇÇØ hemeÀÌ ºÐÇØµÇ¾î »ý¼ºµÇ´Â bilirubin¿¡´Â °­·ÂÇÑ radical º¸Á¶ÀÛ¿ëÀ» ÅëÇÑ Ç× ¿°ÁõÀÛ¿ëÀÌ ÀÖ°í, µ¿½Ã¿¡ »ý¼ºµÇ´Â ÀÏ»êȭź¼Ò´Â Ç÷°üÈ®ÀåÀ» ÃËÁøÇÏ¿© Àå±âÀÇ Ç÷·ù¸¦ À¯ÁöÇÑ´Ù°í º¸°íµÇ°í ÀÖ´Ù. º» Å°Æ®¿¡ Æ÷ÇÔµÈ 2Á¾ÀÇ monoclonal antibody (GTS-1, GTS-3)´Â Ç× Heme Oxygenase 1 Antibody·Î µÎ Ç×ü ¸ðµÎ rat ¹× human Ç׿ø¿¡ ¹ÝÀÀÇÑ´Ù. ÀÌ µÎ Ç×ü´Â Rat Heme Oxygenase 1À» ¹ßÇöÇÏ´Â ÇüÁúÀüȯ ¼¼Æ÷ (WR19LrHO-1)ÀÇ microsomeÀ» ¸é¿ª¿øÀ¸·Î Á¦ÀÛµÈ mouse hybridoma cell À¯·¡ÀÌ´Ù. À̵é 2Á¾ÀÇ Ç×ü¸¦ Á¶ÇÕÇÏ¿© Rat Heme Oxygenase 1À» Á¤·®ÇÒ ¼ö ÀÖ´Â sandwich typeÀÇ ELISAÀ̸ç Human Ç׿ø¿¡ ´ëÇؼ­´Â µÎ Ç×üÀÇ Æ¯¼º»ó ÃøÁ¤ÀÌ ºÒ°¡´ÉÇÏ´Ù.
¿ø¸®
Precoat type 2 Step Sandwich EIA¹ý
¼º´É
ÃøÁ¤ ¹üÀ§ £º 0.125 ~ 8.0 ng/§¢
°ËÃâ °¨µµ £º 0.125 ng/§¢
ƯÀ̼º £º Rat¡¤Heme Oxygenase-1°ú ƯÀÌÀûÀ¸·Î ¹ÝÀÀÇÏ°í,
Rat¡¤Heme Oxygenase-2¿¡´Â ¹ÝÀÀÇÏÁö ¾Ê´Â´Ù.
Human, Rabbit, guinea pig, Mouse´Â ÃøÁ¤ÇÒ ¼ö ¾ø½À´Ï´Ù.
ÃøÁ¤½Ã°£ £º ÃøÁ¤ Á¶ÀÛ 2½Ã°£ 30ºÐ
°Ëü £º Ç÷û, Àå±â ÃßÃâ¹°, ¹è¾ç ¼¼Æ÷»óû
°Ëü·®À¸·Î¼­ 100 §¡/assay wellÀÌ ÇÊ¿ä

Keyword : ½ºÆ®·¹½º,¿°Áõ

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