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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > High input DNA-seq > ThruPLEX¢ç Tag-Seq FLEX
UMI¸¦ Àû¿ëÇÏ¿© Á¤È®ÇÑ FFPE, cell-free DNA NGS library Á¦ÀÛ °¡´É

ThruPLEX¢ç Tag-Seq FLEX

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Clontech
R400734
ThruPLEX¢ç Tag-Seq FLEX
°ü·ÃÇмú ±¸¸ÅÇϱâ new ¶óÀ̼±½º 
24 ȸ
1,478,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX Tag-Seq FLEX User Manual.pdf
Clontech
R400735
ThruPLEX¢ç Tag-Seq FLEX
°ü·ÃÇмú ±¸¸ÅÇϱâ new ¶óÀ̼±½º 
96 ȸ
5,032,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • ThruPLEX¢ç FLEX chemistry ¸¦ ±â¹ÝÀ¸·Î low-frequency variant °ËÃâ ¹× PCR bias ¹æÁö¸¦ À§ÇÑ unique molecular identifiers (UMI) Ãß°¡
  • °£ÆíÇÑ single tube workflow·Î »ùÇà loss ¹æÁö ¹× ½Ã°£ Àý¾à °¡´É
  • Input¾ç¿¡ °ü°è¾øÀÌ ÀçÇö¼ºÀÌ ³ô°í ±ÕÀÏÇÑ Ä¿¹ö¸®Áö ½ÇÇö, GC ÇÔ·®ÀÌ ³ôÀº ¿µ¿ªµµ ±ÕÀÏÇÑ Ä¿¹ö¸®Áö À¯Áö
  • ºÐ¼®ÀÌ ¾î·Á¿î FFPE À¯·¡ DNA, cfDNA µîÀÇ ºÐ¼®À» º¸´Ù ´õ Á¤È®ÇÏ°Ô
  • Unique Dual Index kit (º°µµ±¸¸Å)¿Í °°ÀÌ »ç¿ëÇϸé 384 »ùÇñîÁö multiplexing °¡´É
Á¦Ç° ¼³¸í
º» Á¦Ç°Àº ThruPLEX¢ç FLEX chemistry¸¦ ±â¹ÝÀ¸·Î unique molecular identifiers (UMI) Ãß°¡ÇÏ¿© º¸´Ù ´õ Á¤È®ÇÑ ºÐ¼®ÀÌ °¡´ÉÇϵµ·Ï ÇÏ¿´´Ù. ÃÖ´ë 30 §¡ÀÇ input volumeÀ» Àû¿ëÇÒ ¼ö ÀÖÀ¸¸ç, single tube¿¡¼­ ¾à 2½Ã°£ ¸¸¿¡ °£ÆíÇÑ 3-step workflow·Î ¹ÝÀÀÀÌ °¡´ÉÇÏ´Ù(±×¸² 1). Áß°£ Á¤Á¦ ´Ü°è°¡ ¾ø¾î »ùÇà À̵¿ÀÌ ÇÊ¿ä ¾øÀ¸¹Ç·Î handling error³ª »ùÇà loss¸¦ ¹æÁöÇÒ ¼ö ÀÖ´Ù. ThruPLEX¢ç DNA-Seq FLEX¿Í Unique Dual Index kit (UDI, º°µµ±¸¸Å)¸¦ °°ÀÌ »ç¿ëÇϸé, ÃÖ´ë 384°³ÀÇ »ùÇÃÀ» multiplexing ÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. ThruPLEX¢ç FLEX chemistry : single tube, 3-step workflow
- Stem loop adaptor¿¡ UMI¸¦ Ãß°¡µÇ¾î ÀÖ¾î, º°µµÀÇ Ãß°¡ °úÁ¤¾øÀÌ UMI¸¦ Æ÷ÇÔÇÑ library Á¦ÀÛ °¡´É


±×¸² 2. ThruPLEX¢ç Tag-Seq FLEX ³»Æ÷ÇԵǾîÀÖ´Â 144°³Á¶ÇÕÀÌ°¡´ÉÇÑ unique molecular identifiers (UMIs)
ThruPLEX¢ç Tag-Seq FLEX´Â UMI¸¦ Àû¿ëÇÏ¿© PCR°úÁ¤À̳ª sequencing Áß ¹ß»ýÇÒ ¼ö ÀÖ´Â false positive callÀ» ÁÙ¿© ºÐ¼®ÀÇ Á¤È®¼ºÀ» ¿Ã¸± ¼ö ÀÖµµ·Ï °³¹ßµÇ¾ú´Ù. UMI 7 base´Â ºÐ¼®ÀÇ ¿ëÀ̼ºÀ» ¿Ã¸®±â À§ÇÏ¿© sequencing ½ÃÀۺκп¡ À§Ä¡ÇÑ´Ù.

[Input »ùÇà Á¤º¸]

 

ThruPLEX¢ç Tag-Seq FLEX

Source

Cells, plasma, urine, other biofluids, FFPE, gDNA

Type

Isolated cell-free DNA or fragmented dsDNA

Input ¾ç

1 ng - 200 ng

Input volume

30 §¡

Input buffer

¡Â10 mM Tris, ¡Â0.1 mM EDTA

ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.

Keyword :

High input DNA-seq
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FFPE, cell-free DNA NGS libraryÁ¦ÀÛ¿¡ ÃÖÀû
ThruPLEX¢ç DNA-Seq FLEX
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UMI¸¦ Àû¿ëÇÏ¿© Á¤È®ÇÑ FFPE, cell-free DNA NG..
ThruPLEX¢ç Tag-Seq FLEX