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Home > ÀüÁ¦Ç°º¸±â > NGS °ü·Ã > High input DNA-seq > ThruPLEX¢ç DNA-Seq FLEX
FFPE, cell-free DNA NGS libraryÁ¦ÀÛ¿¡ ÃÖÀû

ThruPLEX¢ç DNA-Seq FLEX

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Clontech
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ThruPLEX¢ç DNA-Seq FLEX
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997,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX DNA-Seq FLEX User Manual.pdf
Clontech
R400736
ThruPLEX¢ç DNA-Seq FLEX
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Clontech
635100
ThruPLEX¢ç DNA-Seq FLEX EF
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1,122,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â ThruPLEX DNA-Seq FLEX EF User Manual.pdf
Clontech
635101
ThruPLEX¢ç DNA-Seq FLEX EF
°ü·ÃÇмú ±¸¸ÅÇϱâ new ¶óÀ̼±½º 
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3,464,000¿ø  Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â

  • °£ÆíÇÑ single tube workflow·Î »ùÇà loss ¹æÁö ¹× ½Ã°£ Àý¾à °¡´É
  • ±Ø¼Ò·® (pg¼öÁØ) fragmented dsDNA¸¸ ÀÖÀ¸¸é Illumina ºÐ¼®¿ë NGS library Á¦ÀÛ °¡´É
    - ThruPLEX¢ç DNA-Seq FLEX : 250 pg - 200 ng
    - ThruPLEX¢ç DNA-Seq FLEX EF : 100 pg - 200 ng
  • Input¾ç¿¡ °ü°è¾øÀÌ ÀçÇö¼ºÀÌ ³ô°í ±ÕÀÏÇÑ Ä¿¹ö¸®Áö ½ÇÇö, GC ÇÔ·®ÀÌ ³ôÀº ¿µ¿ªµµ ±ÕÀÏÇÑ Ä¿¹ö¸®Áö À¯Áö
  • ºÐ¼®ÀÌ ¾î·Á¿î FFPE À¯·¡ DNA, cfDNA µîÀÇ ºÐ¼®¿¡ ÃÖÀû
  • Unique Dual Index kit (º°µµ±¸¸Å)¿Í °°ÀÌ »ç¿ëÇϸé 384 »ùÇñîÁö multiplexing °¡´É
  • DNA ´ÜÆíÈ­ È¿¼Ò(enzymatic fragmentation)¸¦ Æ÷ÇÔÇÑ ThruPLEX¢ç DNA-Seq FLEX EF ¶óÀξ÷
Á¦Ç° ¼³¸í
ThruPLEX¢ç DNA-Seq FLEX chemistry´Â ÃÖ´ë 30 §¡ÀÇ input volumeÀ» Àû¿ëÇÒ ¼ö ÀÖÀ¸¸ç, single tube¿¡¼­ ¾à 2½Ã°£ ¸¸¿¡ °£ÆíÇÑ 3-step workflow·Î ¹ÝÀÀÀÌ °¡´ÉÇÏ´Ù(±×¸² 1). Áß°£ Á¤Á¦ ´Ü°è°¡ ¾ø¾î »ùÇà À̵¿ÀÌ ÇÊ¿ä ¾øÀ¸¹Ç·Î handling error³ª »ùÇà loss¸¦ ¹æÁöÇÒ ¼ö ÀÖ´Ù. ThruPLEX¢ç DNA-Seq FLEX¿Í Unique Dual Index kit (UDI, º°µµ±¸¸Å)¸¦ °°ÀÌ »ç¿ëÇϸé, ÃÖ´ë 384°³ÀÇ »ùÇÃÀ» multiplexing ÇÒ ¼ö ÀÖ´Ù. º» ½Ã½ºÅÛÀ» »ç¿ëÇϸé novo sequencing, whole genome resequencing, whole exome sequencing°ú ´Ù¾çÇÑ enrichment techniquesÀÌ Àû¿ëµÈ high-coverage deep sequencing¿¡¼­ ÃÖ°íÀÇ °á°ú¸¦ ¾òÀ» ¼ö ÀÖ´Ù. Cell-free plasma DNA ȤÀº formalin-fixed paraffin embedded (FFPE) Á¶Á÷¿¡¼­ ÃßÃâÇÑ ¼Õ»óµÈ DNA µî ÀÛÀº fragment DNA ºÐ¼®¿¡ ÃÖÀûÀÎ Á¦Ç°ÀÌ´Ù.
ThruPLEX¢ç DNA-Seq FLEX EF´Â ThruPLEX¢ç DNA-Seq FLEX chemistry¿¡ ÀýÆíÈ­ ±â´ÉÀ» Ãß°¡ÇÑ Á¦Ç°ÀÌ´Ù. Á¦Ç°¿¡ Æ÷ÇԵǾî ÀÖ´Â ThruPLEX¢ç FLEX Enzymatic Fragmentation ModuleÀº ThruPLEX¢ç DNA-Seq FLEX repair °úÁ¤¿¡¼­, È¿¼Ò¸¦ ÀÌ¿ëÇÏ¿© ÀýÆíÈ­ ½ÃÅ°¸ç, Àý´ÜµÇ´Â ÀýÆí Å©±âµµ Á¶ÀýÇÒ ¼ö ÀÖµµ·Ï ¼³°èµÇ¾î ÀÖ´Ù. À̸¦ ÅëÇØ ±â°èÀûÀÎ ÀýÆíÈ­ (mechanical fragmentation) ȤÀº º°µµÀÇ È¿¼Ò¸¦ ÀÌ¿ëÇÑ ÀýÆíÈ­ °úÁ¤À» »ý·«ÇÒ ¼ö ÀÖ´Ù.


±×¸² 1. ThruPLEX¢ç DNA-Seq FLEX¸¦ ÀÌ¿ëÇÑ single tube, 3-step workflow


±×¸² 2. ThruPLEX¢ç DNA-Seq FLEX ±â¼ú
Stem-loop adaptor¸¦ repair °úÁ¤¿¡¼­ bluntµÈ DNAÀÇ 5¡¯ ¸»´Ü¿¡ ligation ½ÃŲ ÈÄ, high-fidelity polymerase·Î barcode°¡ Æ÷ÇÔµÈ Illumina NGS library¸¦ Á¦ÀÛÇÔ.

[Input »ùÇà Á¤º¸]

 

ThruPLEX¢ç DNA-Seq FLEX

ThruPLEX¢ç DNA-Seq FLEX EF

Nucleic acid

Fragmented double-stranded DNA or cfDNA

Double-stranded DNA

Source

Cells, plasma, urine, other biofluids, FFPE, tissues, fresh tissues, frozen tissues

Cells, fresh tissues, frozen tissues, microbes

Type

Mechanically sheared; enzymatically fragmented; low-molecular-weight cell-free DNA

Á¦Ç°¿¡ Æ÷ÇÔµÈ ThruPLEX¢ç FLEX Enzymatic Fragmentation Module ·Î ÀýÆíÈ­ÇÏ¿© Àû¿ë

Molecular size

<1,000 bp

Input ¾ç

250 pg - 200 ng

100 pg - 200 ng

Input volume

30 §¡

Input buffer

¡Â10 mM Tris, ¡Â0.1 mM EDTA


[Âü°í]
ThruPLEX FLEX outperforms NEBNext Ultra II for DNA-seq of cell-free and FFPE DNA
(ºñ±³ µ¥ÀÌÅ͸¦ È®ÀÎÇÏ·Á¸é, Ŭ¸¯Çϼ¼¿ä)
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.

Keyword :

High input DNA-seq
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FFPE, cell-free DNA NGS libraryÁ¦ÀÛ¿¡ ÃÖÀû
ThruPLEX¢ç DNA-Seq FLEX
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