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Bacteria Genomic DNA Extraction Kit Ver.3.0

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
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Takara
9763A
TaKaRa MiniBEST Bacteria Genomic DNA Extraction Kit Ver.3.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
(Package)
194,000¿ø  9763_e.v1309Da.pdf
Takara
9763B
TaKaRa MiniBEST Bacteria Genomic DNA Extraction Kit Ver.3.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9763A x 4)
700,000¿ø 


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TaKaRa MiniBEST Bacteria Genomic DNA Extraction Kit Ver.3.0Àº gram-positive/negative bacteria¸¦ Æ÷ÇÔÇÑ ´Ù¾çÇÑ bacteriaÀÇ genomic DNA¸¦ ÃßÃâÇÏ´Â Á¦Ç°ÀÌ´Ù. ƯÈ÷ DNA Preparation membrane°ú Ưº°ÇÏ°Ô Á¦ÀÛµÈ lysis buffer¸¦ »ç¿ëÇÔÀ¸·Î½á bacteria·ÎºÎÅÍ genomic DNA¸¦ È¿À²ÀûÀ¸·Î ÃßÃâÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇϸé cell lysis ÀÌÈÄ 20ºÐ¸¸¿¡ °í¼øµµÀÇ genomic DNAÀÇ ºÐ¸®°¡ °¡´ÉÇϸç, 1.0 - 5.0 x 109 bacteria (OD600 = 1, equivalent to 1ml broth containing 1.0 x 109 cells)·ÎºÎÅÍ 1 - 20ugÀÇ °í¼øµµÀÇ genomic DNA¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© ÃßÃâÇÑ genomic DNA´Â PCR, Southern blotting, RAPD, AFLP, RFLP³ª ±× ¿Ü ºÐÀÚ»ý¹°ÇÐ ½ÇÇè¿¡ Àû¿ëÇÒ ¼ö ÀÖ´Ù.
Flowchart
³»¿ë
º» Kit´Â Part I°ú Part II·Î ±¸¼ºµÇ¾î ÀÖ´Ù.

¢º Part I
Proteinase K (20 mg/ml)

1 ml

Lysozyme (20 mg/ml)*1

1.25 ml x 2

RNaseA (10 mg/ml)

500 ul


*1: Avoid repeated freezing and thawing. Once thawed, store at 4¡É for a long time.

¢º Part II
Buffer BS

28 ml

Buffer GL*1

12 ml

Buffer GB*1

12 ml

Buffer WA*1

28 ml

Buffer WB*2

24 ml

Elution Buffer

14 ml

Spin Column

50

Collection tube

50


*1: Contains strong denaturant. Be careful to avoid contacting with skin and eyes. In the case of such contact, wash immediately with plenty
     water and seek medical advice.
*2: Before using the kit, add 56 ml of 100% ethanol. Mix well.

¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% Ethanol
2. Sterilized water
º¸°ü ¹× ¿î¼Û
1. Part I should be shipped and stored at -20¡É.
2. Part II can be stored and shipped at room temperature (15 - 25¡É).

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