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Viral RNA/DNA Extraction column

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
9766A
TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
50 ȸ
(Package)
230,000¿ø  9766_e.v2005Da.pdf
Takara
9766B
TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ (9766A x 4)
832,000¿ø 


»ó¼¼Á¤º¸
TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0Àº plasma, whole blood, cell-free body fluids, virus stock solution, infected tissue¸¦ Æ÷ÇÔÇÑ ´Ù¾çÇÑ sample source·ÎºÎÅÍ virus RNA/DNA ¸¦ Á¤Á¦ÇÏ´Â Á¦Ç°ÀÌ´Ù. º» Á¦Ç°Àº biological sample·ÎºÎÅÍ viral RNA/DNA¸¦ È¿À²ÀûÀ¸·Î Á¤Á¦ÇÒ ¼ö ÀÖ´Â nucleic acid preparation membrane°ú Ưº°ÇÑ lysis buffer·Î ±¸¼ºµÇ¾îÀÖ´Ù.
º» Á¦Ç°À» ÀÌ¿ëÇϸé virus lysis ÀÌÈÄ, 20ºÐ¸¸¿¡ °í¼øµµÀÇ RNA/DNA¸¦ Á¤Á¦ÇÒ ¼ö ÀÖ´Ù. Flury strain rabies virus·ÎºÎÅÍ 1.6 x 105 copies, Hepatitis A virus·ÎºÎÅÍ 1.0 x 104 copiesÀÇ RNA¸¦ ÃßÃâ °¡´ÉÇÏ´Ù. º» Á¦Ç°À» ÀÌ¿ëÇÏ¿© ÃßÃâÇÑ RNA´Â RT-PCR, Northern blotting°ú °°Àº ¿©·¯ ½ÇÇè¿¡ Àû¿ë °¡´ÉÇÏ´Ù.
Flowchart
³»¿ë
¢º Part I
Proteinase K (20 mg/ml)

1 ml

Carrier RNA

50 ul



¢º Part II
Buffer VGB

12 ml

Buffer RWA*1

28 ml

Buffer RWB*2

24 ml

RNase free dH2O

2 ml x 2

Spin Columns

50

Collection tubes

50

RNase free collection tube (1.5 ml)

50


£ª1: Contains Strong denaturant. Be careful to avoid contacting with skin and eyes. In the case of such contact, wash immediately with plenty
      water and seek medical advice.
£ª2: Before using the kit, add 56 ml of 100% ethanol. Mix well.

¡Ø ÀÌÇÏ ½Ã¾àÀº º°µµ Áغñ°¡ ÇÊ¿äÇÕ´Ï´Ù.
1. 100% ethanol
2. PBS solution
º¸°ü ¹× ¿î¼Û
1. Part I can be stored at -20¡É, and part II of the kit can be stored at room temperature (15-25¡É).
2. Part I can be shipped at -20¡É, and part II of the kit can be shipped at room temperature (15-25¡É).

Keyword :

Total RNA ÃßÃâ ¡¤ Á¤Á¦
>
gDNA Eraser Column»ç¿ëÀ¸·Î, ´Ù¾çÇÑ »ùÇÿ¡¼­ ..
Universal RNA Extraction Kit
>
2Á¾·ùÀÇ lysis buffer·Î ´Ù¾çÇÑ ½Ä¹° »ùÇÿ¡¼­ ..
Plant RNA Extraction Kit
>
Á¶Á÷ ¡¤ ¹è¾ç¼¼Æ÷·Î ºÎÅÍ RNA ÃßÃ⠽þà
RNAiso Plus
>
Ç÷¾× µî ¼öºÐÀÌ ¸¹Àº »ùÇÃÀÇ RNA ÃßÃâ
RNAiso Blood
>
´Ù´ç·ù ÇÔÀ¯»ùÇà Àü󸮽þà
Fruit-mate¢â for RNA Purification
>
½Ä¹° À¯·¡ RNA »ùÇÿ¡¼­ ´Ù´ç·ù Á¦°Å
High-Salt Solution for Precipitation (Plant)
>
¾×üÁú¼Ò°¡ ÇÊ¿ä¾ø´Â È¿¸ð Àüó¸® ½Ã¾à
Yeast Processing Reagent (for total RNA preparation)