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°í¼Ó PCRÀ» À§ÇÑ

Fast PCR¿ë Z-Taq

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
R006A
TaKaRa Z-Taq¢â
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 U
277,000¿ø  R006A_e.v1411Da.pdf
Takara
R006B
TaKaRa Z-Taq¢â
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 U (R006A x 4)
996,000¿ø 

Á¦Ç°¼³¸í
º» Á¦Ç°Àº °í¼Ó PCR¿ëÀ¸·Î °³¹ßµÈ DNA polymerase·Î rTaq DNA polymeraseº¸´Ù ¾à 5¹è ºü¸¥ PCR ÁõÆø¼Óµµ·Î 1 kb ´ÜÆíÀÇ ÁõÆøÀ» ¾à 20ºÐ¸¸¿¡ ¿Ï·áÇÒ ¼ö ÀÖÀ¸¸ç, ¶Ù¾î³­ ¹ÝÀÀ¼ºÀ¸·Î human genomic DNA¸¦ ÁÖÇüÀ¸·Î 17.5 kb±îÁö ÁõÆøÇÒ ¼ö ÀÖ´Ù.
¹ÝÀÀ ½Ã°£À» ÁÙÀÏ ¼ö ÀÖ¾î ´Ù¼öÀÇ »ùÇà 󸮿¡ À¯¿ëÇϸç, cycle¼ö¸¦ Áõ°¡½ÃÅ´À¸·Î¼­(~ 50 cycles) PCR °¨µµµµ Çâ»ó½Ãų ¼ö ÀÖ´Â °í¼Ó PCR¿ë È¿¼ÒÀÌ´Ù.


TaKaRa Z-Taq¢â (Code R006A, 200 U)
1.

TaKaRa Z-Taq

200 U

2.

10¡¿Z-Taq Buffer (Mg2+plus) (30 mM)

800 §¡

3.

dNTP Mixture

800 §¡

º¸Á¸ -20¡É
Çü»ó

20 mM

Tris-HCl(pH8.0)

100 mM

KCl

0.1 mM

EDTA

1 mM

DTT

0.5£¥

Tween 20

0.5£¥

Nonidet P-40

50£¥

Glycerol

ÁõÆø »ê¹°ÀÇ ¸»´Ü
TaKaRa Z-Taq¢âÀ» ÀÌ¿ëÇØ ÁõÆøÇÑ PCR »ê¹°ÀÇ ´ëºÎºÐÀº 3£§¸»´Ü¿¡ A°¡ 1 ¿°±â ºÎ°¡µÇ¾î ÀÖ´Ù. µû¶ó¼­ PCR »ê¹°À» Á÷Á¢ T-Vector (pMD20 (Code 3270), pMD19 (Simple) (Code 3271) µî) ¶Ç´Â Mighty TA-cloning Kit (Code 6028)À» ÀÌ¿ëÇÏ¿© cloningÇÒ ¼ö ÀÖ´Ù.
¶Ç, Mighty Cloning Reagent Set (Blunt End) (Code 6027)·Î ÆòÈ° ¸»´ÜÈ­ ¹× ÀλêÈ­¸¦ 󸮸¦ ÇÏ¿©, ÆòÈ° ¸»´Ü vector¿¡µµ cloningÇÒ ¼ö ÀÖ´Ù.

Keyword :

Standard PCR: Ex, LA, rTaq
>
1st choice PCR È¿¼Ò
TaKaRa Ex Premier¢â DNA Polymerase
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Ex Taq Hot Start Version
>
°íÈ¿À², °í°¨µµ – PCR È¿¼ÒÀÇ standard
TaKaRa Ex Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
LA Taq Hot Start Version
>
±ä ´ÜÆíÀÇ DNA / GC rich ¼­¿­ ÁõÆø
LA Taq
>
Hot start PCR·Î ºñƯÀÌÀûÀÎ ÁõÆøÀ» ¹æÁö
Taq Hot Start Version
>
Recombinant Taq DNA Polymerase
Recombinant Taq
>
½Ç¿Â º¸°ü °¡´ÉÇÑ master mix
µ¿°á°ÇÁ¶ ºÐÁÖÇüÅÂ-Taq HS Premix
>
Fast PCR¿ë hot start È¿¼Ò
SpeedSTAR HS È¿¼Ò
>
°í¼Ó PCRÀ» À§ÇÑ
Fast PCR¿ë Z-Taq
>
Hot Start PCR ¿ë Anti-Taq Antibody
Taq Antibody
>
»ç¿ëÀÌ Æí¸®ÇÑ
Premix ½Ã¾à (Ex, LA Taq)
>
2¡¿ Premix PCR Solution
Premix ½Ã¾à (Hot Start Version)