´Ý±â
TaKaRa
Á¦Ç°¸í/Á¦Ç°ÄÚµå
Å°¿öµå°Ë»ö
°Ë»ö
´Ý±â
  • °í°´Áö¿ø 
  • ¾÷¹«¾È³»¦¢Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢02-2081-2510
  • Email     ¦¢[email protected]
  • ´ëÀüÁö»ç 
  • ¾÷¹«¾È³»¦¢´ëÀü/ÃæûÁö¿ª ÁÖ¹®, Á¦Ç°¹®ÀÇ
  • ÀüÈ­¹øÈ£¦¢042-828-6525
  • Email     ¦¢[email protected]
  • ¾÷¹«½Ã°£¾È³»
  • [ Æò¡¡¡¡ÀÏ ] 09 : 00 ~ 18 : 00 ¦¢ [ Á¡½É½Ã°£ ] 12 : 00 ~ 13 : 00
  • Å䡤ÀÏ¿äÀÏ, °øÈÞÀÏÀº ÈÞ¹«ÀÔ´Ï´Ù.
Home > ÀüÁ¦Ç°º¸±â > Epigenetics > DNA Methlyation ¿¬±¸ > TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖÀû

TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)

-

Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Takara
RR071A
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
200 ȸ
(50 §¡ ¹ÝÀÀ°è)
321,000¿ø  RR071_v1901.pdf


  • µ¶ÀÚÀûÀΠ÷°¡ ÀÎÀÚ·Î GCÇÔ·® 60~70%ÀÇ Å¸°Ù¿¡¼­µµ ¹ÝÀÀ¼ºÀÌ Å©°Ô Çâ»ó
  • GCÇÔ·® 60% ¹Ì¸¸ÀÇ Å¸°Ù¿¡¼­µµ ¾çÈ£ÇÏ°Ô ¹ÝÀÀÇϹǷΠµ¿ÀÏ Á¶°ÇÀ¸·Î ¹ßÇö Çؼ®ÀÌ °¡´É
  • CpG island¸¦ Æ÷ÇÔÇÑ GC rich¿µ¿ªÀÇ Çؼ®¿¡µµ Ãßõ
Á¦Ç°¼³¸í
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)´Â TB Green¢çÀ» ÀÌ¿ëÇÑ intercalating ¹ý Real Time PCR Àü¿ë ½Ã¾àÀÌ´Ù. 2¡¿ ³óµµÀÇ premix type½Ã¾àÀ¸·Î, ½Ç½Ã°£ ¸ð´ÏÅ͸µ¿¡ ÀûÇÕÇÑ ³óµµÀÇ TB Green¢çÀ» ¹Ì¸® Æ÷ÇÔÇÏ°í ÀÖ¾î ¹ÝÀÀ ¾×ÀÇ Á¶Á¦°¡ °£´ÜÇÏ´Ù.
º» Á¦Ç°Àº ´ÙÄ«¶óÀÇ µ¶ÀÚÀûÀΠ÷°¡Á¦¿Í ¹ÝÀÀ Á¶¼ºÀÇ °³·®À¸·Î GC ÇÔ·® 60-70%ÀÇ GC rich ¿µ¿ª¿¡ ´ëÇÑ ¹ÝÀÀ¼º°ú Á¤·®¼ºÀÌ Å©°Ô Çâ»óµÇ¾ú´Ù (ÁõÆø»çÀÌÁî: ~200 bp ±ÇÀå). º» Á¦Ç°À» ÀÌ¿ëÇÔÀ¸·Î½á CpG island¸¦ Æ÷ÇÔÇÑ ¿µ¿ª µî GC rich Ÿ°Ù¿¡¼­µµ Á¤¹ÐÇÑ Çؼ®ÀÌ °¡´ÉÇÏ´Ù. GCÇÔ·® 60% ÀÌÇÏÀÇ ÀϹÝÀûÀΠŸ°Ù¿¡¼­µµ ¹ÝÀÀ¼ºÀÌ ¾çÈ£ÇϹǷÎ, µ¿ÀÏÇÑ PCR Á¶°ÇÀ¸·Î Á¤·®ºÐ¼® ÇÒ ¼ö ÀÖ´Â ÀåÁ¡ÀÌ ÀÖ´Ù.
³»¿ë
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time) (Code RR071A, 50 §¡ ¹ÝÀÀ½Ã 200ȸ ºÐ·®)

1.

TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time) (2¡¿conc.)*1

1 §¢¡¿ 5°³

2.

ROX Reference Dye (50¡¿conc.)*2

200 §¡

3.

ROX Reference Dye II (50¡¿conc.)*2

200 §¡


*1 TaKaRa Ex Taq HS, dNTP Mixture, Mg2£« ¹× TB Green¢çÀ» Æ÷ÇÔÇÑ´Ù.
*2 Life TechnologiesÀÇ Real Time PCR ÀåÄ¡ µî well °£ Çü±¤ ½ÅÈ£¸¦ º¸Á¤ÇÏ´Â ÀåÄ¡·Î Çؼ®ÇÏ´Â °æ¿ì¿¡ »ç¿ëÇÑ´Ù.
  • ROX Reference Dye(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7300 Real-Time PCR System (Life Technologies)
    StepOnePlus Real-Time PCR System (Life Technologies)
  • ROX Reference Dye II(50¡¿)À» ÷°¡ÇÏ´Â ±âÁ¾
    Applied Biosystems 7500/7500 Fast Real-Time PCR System (Life Technologies)
  • ÷°¡°¡ ÇÊ¿ä ¾ø´Â ±âÁ¾
    CronoSTAR¢â 96 Real-Time PCR System (4ch) (Code 640231)
    Thermal Cycler Dice Real Time System III (Code TP950/TP970/TP980)
    Thermal Cycler Dice Real Time System II (Code TP900/TP960)
    Thermal Cycler Dice Real Time System Single (Code TP850/TP870)
    Thermal Cycler Dice Real Time System Lite (Code TP700/TP760)
    Smart Cycler System/Smart Cycler II System (Cepheidȍ)
    LightCycler 96/LightCycler 480 System (Roche Diagnosticsȍ)
    CFX96 Real-Time PCR Detection System (Bio-Radȍ)
º¸Á¸
4¡É º¸Á¸: 6°³¿ù ¾ÈÁ¤
¡Ø ¹Ýµå½Ã Â÷±¤ÇϽʽÿÀ. ¶ÇÇÑ ¿À¿°¿¡ ÁÖÀÇÇϽʽÿÀ.

- Àå±â º¸Á¸ÀÇ °æ¿ì´Â -80 ¡É¿¡¼­ º¸°üÇϽʽÿÀ. (-20 ¡É ÀúÀåÇÏÁö ¸¶½Ê½Ã¿À.)
- ÀÏ´Ü ¿ëÇØ ÈÄ¿¡´Â 4¡É º¸°üÇÏ°í, 6°³¿ù¾È¿¡ »ç¿ëÇÒ °ÍÀ» ±ÇÀåÇÕ´Ï´Ù.

Keyword :

DNA Methlyation ¿¬±¸
>
Bisulfite ó¸® ÈÄÀÇ DNA PCR ÁõÆø¿¡ ÃÖÀû
TaKaRa EpiTaq HS (for bisulfite-treated DNA)
>
ƯÁ¤ ¿µ¿ªÀÇ DNA MethylationÀ» Çؼ® °¡´É
EpiScope¢ç MSP Kit
>
genomic DNA ¶Ç´Â CpG island ¿µ¿ªÀÇ ÁõÆø¿¡ ÃÖ..
TB Green¢ç Premix Ex Taq¢â GC (Perfect Real Time)
>
DNA ¸ÞÆ¿È­ Çؼ® ÄÁÆ®·Ñ
¸ÞÆ¿È­/ºñ¸ÞÆ¿È­ ÄÁÆ®·Ñ °Ô³ð DNA