구성
용량
Cellartis DEF-CS 500 Basal Medium
500 ml
Cellartis iPSC Single-Cell Cloning DEF-CS COAT-1
800 ㎕ x 2
Cellartis iPSC Single-Cell Cloning DEF-CS additives
1 set
DEF-CS GF-1
750 ㎕ x 2
DEF-CS GF-2
500 ㎕
DEF-CS GF-3
a. Guide-it CRISPR/Cas9 Gesicle Packaging Set (Code 632616)
Guide-it CRISPR/Cas9 Gesicle Packaging Mix 1(green cap) Guide-it CRISPR/Cas9 Gesicle Packaging Mix 2(yellow cap) A/C Heterodimerizer(500 nM in Ethanol) Protamine Sulfate(4 mg/ml)
10 vials 10 vials 50 ㎕ 200㎕
b. pGuide-it-sgRNA1 Vector(Linear)(7.5 ng/㎕) c. Guide-it Ligation Components v2
20 ㎕
DNA Ligation Mighty Mix Guide-it Oligo Annealing Buffer Guide-it Control Annealed Oligos v2(100 fmol/㎕) Guide-it Sequencing Primer 1(100 pmol/㎕) PCR-Grade Water
50 ㎕ 1.5 ml 10 ㎕ 10 ㎕ 1 ml
d. Stellar Competent Cells (Code 636763)
Stellar Competent Cells(100㎕/tube) SOC Medium(1 ml/tube) pUC19 Vector(0.1 ng/㎕)
10 tubes 10 tubes 10 ㎕
2.
Guide-it Recombinant Cas9 (Electroporation-Ready) (Code 632641)
Guide-it Recombinant Cas9 (Electroporation-Ready)
100 ㎕
3.
Guide-it sgRNA In Vitro Transcription Kit (Code 632635)
Guide-it Scaffold Template (1 ng/㎕) Guide-it In Vitro Transcription Buffer RNase Free Water Guide-it T7 Polymerase Mix (33 U/㎕) PrimeSTAR Max Premix (2X) Recombinant DNase I (RNase-Free) (5 U/㎕)
50 ㎕ 350 ㎕ 1 ml 150 ㎕ 625 ㎕ 100 ㎕
b. Guide-it IVT RNA Clean-Up Kit（Code632638)
RNase Free Water IVT Binding Buffer IVT RNA Clean-Up Spin Column IVT Wash Buffer Collection Tubes (2 ml)
13 ml 3 ml 50회 6 ml x 2 50 회
