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Cellartis
Y40000
RHB Basal
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500 ml
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RHB-A ¹èÁö´Â »óÇÇ ¼ºÀåÀÎÀÚ(EGF)¿Í ¼¶À¯¾Æ¼¼Æ÷ ¼ºÀåÀÎÀÚ-2 (FGF-2)¸¦ ÷°¡ÇÏ¿© »ç¿ëÇÔÀ¸·Î½á ¹«Ç÷û Á¶°Ç¿¡¼­ Á¢Âø¹è¾ç »óÅÂÀÇ ½Å°æÁٱ⼼Æ÷(NSC)ÀÇ À¯Áö¿Í È®´ë ¹è¾ç¿¡ »ç¿ëÇÒ ¼ö ÀÖ´Ù. (Âü°í ¹®Çå 1-5, 9)
¼ºÀå ÀÎÀÚ¸¦ Æ÷ÇÔÇÏ´Â º» ¹èÁö¿¡¼­ ½Å°æ Áٱ⼼Æ÷(NSC)¸¦ ¹è¾çÇÏ¿©, À̹èü ÇÙÇüÀ» À¯ÁöÇÑ »óÅ¿¡¼­ 100 ¼¼´ë ³Ñ¾î±îÁö ½Å°æ¿ø ¼º´ÉÀ» À¯Áö ÇÒ ¼ö ÀÖ´Â °ÍÀÌ ³ªÅ¸³µ´Ù. ¶ÇÇÑ EGF¿Í FGF-2¸¦ ÷°¡Çؼ­, cloneÀÌ ¿ø·¡ °¡Áö°í ÀÖ´Â ¼ºÁúÀÇ ½Å°æ ÁÙ±â(NS)¼¼Æ÷ À¯µµ¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù. (Âü°í ¹®Çå 6)

¸¶¿ì½º ES ¼¼Æ÷ÀÇ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­´Â ¼ºÀå ÀÎÀÚ¸¦ °¡ÇÏÁö ¾Ê°í RHB-A¸¦ »ç¿ëÇÑ´Ù. NDiff 227 (Code Y40002)º¸´Ù È¿°úÀûÀ¸·Î ºÐÈ­°¡ ÃËÁøµÈ´Ù. (Âü°í ¹®Çå 7, 8) °Ô´Ù°¡, ¼ºÀå ÀÎÀÚ¸¦ ÷°¡ÇÏÁö ¾ÊÀº ¹èÁö¿¡¼­ ½Å°æ ÁÙ±â (NS) ¼¼Æ÷¸¦ Á¢Âø ¹è¾çÇϸé, ±â´ÉÀûÀ¸·Î ½Å°æ ¼¼Æ÷·Î ºÐÈ­ ÇÒ ¼ö ÀÖ´Ù. (Âü°í ¹®Çå 1)
In vitro»ó¿¡¼­ human brainÀÇ primary culture radial glia·ÎºÎÅÍ ÃßÃâÇÑ ½Å°æ ÁÙ±â (NS) ¼¼Æ÷ÀÇ È®´ë ¹è¾ç, ºÐÈ­¿¡µµ »ç¿ëÇÒ ¼ö ÀÖ´Ù. (Âü°í ¹®Çå 9)

RHB-Basal ¹èÁö´Â ½Å°æ ÁÙ±â(NS) ¼¼Æ÷ÀÇ Á¢Âø ¹è¾ç½Ã Áõ½Ä ¹× ºÐÈ­¸¦ À§ÇÑ ±âº» ¹èÁöÀÌ´Ù. ¼ºÀå ÀÎÀÚ¿Í ½Å°æ ¼¼Æ÷ÀÇ Ã·°¡ ÀÎÀÚ´Â Æ÷ÇԵǾî ÀÖÁö ¾Ê½À´Ï´Ù. µû¶ó¼­, ¼¼Æ÷ÀÇ Á¾·ù¿¡ µû¶ó ÇÊ¿äÇÑ Ãß°¡ ¿ä¼Ò¸¦ ÷°¡ÇÏ¿© »ç¿ëÇÒ ¼ö ÀÖ´Ù.
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  • ¸¶¿ì½º, human ½Å°æ ÁÙ±â(NS)¼¼Æ÷ Á¢Âø ¹è¾ç¿¡¼­ÀÇ À¯Áö, È®´ë ¹è¾ç
  • ¸¶¿ì½º, ES ¼¼Æ÷ÀÇ ´ÜÃþ ¹è¾ç¿¡¼­ÀÇ ½Å°æ ºÐÈ­
  • ¸¶¿ì½º, human ES ¼¼Æ÷, žÆ, ¼ºÀÎ Á¶Á÷¿¡¼­ ½Å°æ ÁÙ±â (NS) ¼¼Æ÷ÀÇ À¯µµ
  • ¸¶¿ì½º, human ½Å°æ ÁÙ±â (NS) ¼¼Æ÷·Î ºÎÅÍ ½Å°æ ¼¼Æ÷·ÎÀÇ ºÐÈ­
  • Human glioblastoma stem cell À¯Áö, È®´ë ¹è¾ç

Tech Note
A fully defined, serum-free culture system for neural stem cell maintenance and differentiation
Âü°í ¹®Çå
1.   The tumor suppressor microRNA, miR-124a, is regulated by epigenetic silencing and by the transcriptional factor, REST in glioblastoma.
     Tivnan A, et al. Tumour Biol. 2014 Feb;35(2):1459-65.
2.   A cell-penetrating peptide based on the interaction between c-Src and connexin43 reverses glioma stem cell phenotype.
     Gangoso E, et al. Cell Death Dis. 2014 Jan 23;5:e1023
3.   Citrullination regulates pluripotency and histone H1 binding to chromatin
     Christophorou MA, et al. Nature. 2014 Mar 6;507(7490):104-8
4.   Generation and Characterization of a Novel Mouse Embryonic Stem Cell Line with a Dynamic Reporter of Nanog Expression
     Abranches E, et al. PLoS One. 2013;8(3):e59928
5.   Widespread resetting of DNA methylation in glioblastoma-initiating cells suppresses malignant cellular behaviour in a lineage-dependent manner
     Stricker SH, et al. Genes Dev. 2013 Mar 15;27(6):654-69
6.   The non-coding snRNA 7SK controls transcriptional termination, poising, and bidirectionality in embryonic stem cells.
     Castelo-Branco G, et al. Genome Biol. 2013;14(9):R98.
7.   Reproducible culture and differentiation of mouse embryonic stem cells using an automated microwell platform.
     Hussain W, et al. Biochem Eng J. 2013 Aug 15;77(100):246-257.
8.   Current progress for the use of miRNAs in glioblastoma treatment.
     Tivnan A, et al. Mol Neurobiol. 2013 Dec;48(3):757-68
9.   Non-immortalized human neural stem (NS) cells as a scalable platform for cellular assays.
     Hook L, et al. Neurochem Int. 2011 59(3): 432-44.
10.  Hypoxia enhances proliferation of mouse embryonic stem cell-derived neural stem cells.
      Fernandes et al. Biotechnology and Bioengineering 2010 106: 260-270.

Keyword :

Mouse stem cell °ü·Ã Á¦Ç°
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Mouse ESC/iPSC ¹è¾ç¹èÁö (3i technology)
Cellartis¢ç 3i mESC/iPSC Culture Medium
>
Mouse ESC/iPSC ¹è¾ç¹èÁö (2i Technology)
Cellartis¢ç 2i mESC/iPSC Culture Medium
>
È¿À²ÀûÀÎ ¸¶¿ì½º/ human ½Å°æÁٱ⼼Æ÷ÀÇ À¯Áö¿Í..
RHB-A/RHB Basal
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¸¶¿ì½º ES ¼¼Æ÷ ½Å°æºÐÈ­¹èÁö
NDiff227
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Human, mouse ES ¼¼Æ÷¿Í ½Å°æ Áٱ⼼Æ÷ ¹è¾ç¿¡ ..
NDiff N2
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°ñ¾Æ¼¼Æ÷ ºÐÈ­ ½Ã¾à
Osteoblast-Inducer Reagent (for animal cell)