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> pAUR123 DNA
È¿¸ð·Î ´Ü¹éÁú ¹ßÇöÇü Shuttle Vector
pAUR123 DNA
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Takara
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pAUR123 DNA (È¿¸ð ¹ßÇöÇü Vector)
20 ¥ìg
469,000¿ø
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È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
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pAUR123 DNA
pAUR135 DNA
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M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
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¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
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Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
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GenBank
Accession No. AB012284
Á¦Ç°¼³¸í
º» Á¦Ç°Àº ÀÚÀ² º¹Á¦Çü º¤ÅÍ pAUR112 À¯·¡ÀÇ ´Ü¹éÁú ¹ßÇö¿ë plasmid vector·Î¼
Saccharomyces cerevisiae
³»¿¡¼ plasmid »óÅ·Π¾ÈÁ¤ÇÏ°Ô À¯ÁöÇÒ ¼ö ÀÖ´Â È¿¸ð shuttle vectorÀÌ´Ù. ¹ßÇö¿ë promoter·Î¼ ±¸¼ºÀûÀ¸·Î ¹ßÇöÇÏ´Â alcohol dehydrogenase I À¯ÀüÀÚ (
ADH
1)ÀÇ promoter¸¦ ÇÔÀ¯ÇÏ°í ÀÖ´Ù. º» vector´Â ´ëÀå±ÕÀÇ ¼±Åà ¸¶Ä¿·Î ampicillin ³»¼ºÀ¯ÀüÀÚ¸¦, ¶Ç
S. cerevisiae
ÀÇ ¼±Åà ¸¶Ä¿·Î Aurobasidin A (AbA) ³»¼º À¯ÀüÀÚ
AUR
1-C¸¦ °®°í ÀÖÀ¸¸ç ¾ÈÁ¤ÀûÀÎ ÀÚÀ²º¹Á¦¿¡ ÇÊ¿äÇÑ
CEN/ARS
¼¿À» °¡Áö°í ÀÖ´Ù. ¹ßÇöÇÏ°íÀÚ ÇÏ´Â À¯ÀüÀÚ¸¦ °³½Ã codon ATG¸¦ ÇÔÀ¯ÇÑ ÇüÅ·Πcloning site¿¡ »ðÀÔÇÔÀ¸·Î½á È¿¸ð ³»¿¡¼ ±¸¼ºÀûÀ¸·Î ¹ßÇöÇÒ ¼ö ÀÖ´Ù. ¶Ç cloning site¿¡´Â reading frameÀ» shift½ÃŲ 3°³ÀÇ stop codonÀÌ µé¾î ÀÖ´Ù.
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6,982 bp
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Ç×»ý¹°Áú Aureobasidin A¸¦ ÀÌ¿ëÇÑ È¿¸ðÀÇ ÇüÁúÀüȯ¿ë º¤ÅÍ(´Ü¹éÁú ¹ßÇöÇü shuttle vector). È¿¸ð ³»¿¡¼ plasmid »óÅ·Πº¸À¯¡¤ À¯ÁöµÈ´Ù.
»ç¿ë»óÀÇ ÁÖÀÇ
º» vector¿¡ »ç¿ëÇÏ´Â Aph1 promoter´Â °í¹ßÇö ÇüÅÂÀÇ promoter°¡ ¾Æ´Ï¹Ç·Î ¸ñÀû ´Ü¹éÁúÀÇ °í¹ßÇöÀº ±â´ëÇÒ ¼ö ¾ø´Ù.
License Notice : [M11]
pAUR123 DNAÀÇ ±¸Á¶
±×¸² pAUR123 DNA Á¦ÇÑÈ¿¼Ò ¸Ê ¹× cloning site
AUR1-C
S. cerevisiaeÀÇ AbA ³»¼º À¯ÀüÀÚ
PADH1
S. cerevisiaeÀÇ ADH1 À¯ÀüÀÚÀÇ promoter
TADH1
S. cerevisiaeÀÇ ADH1 À¯ÀüÀÚÀÇ terminator
ARS
S. cerevisiaeÀÇ º¹Á¦ ±âÁ¡
CEN
S. cerevisiaeÀÇ centromere
Amp
r
E. coli¿¡¼ÀÇ ¼±Åà ¸¶Ä¿
Ori
E. coli¿¡¼ÀÇ º¹Á¦ ±âÁ¡(pBR322 À¯·¡)
Keyword :
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
Chromosomal Integrating È¿¸ð Suttle Vector
pAUR101 DNA
È¿¸ð ÀÚÀ²º¹Á¦Çü Suttle Vector
pAUR112 DNA
È¿¸ð·Î ´Ü¹éÁú ¹ßÇöÇü Shuttle Vector
pAUR123 DNA
Ãâ¾Æ È¿¸ð ¿ë Marker Á¦°ÅÇü Vector
pAUR135 DNA
Schizo. pombe ¿ë ´Ü¹éÁú ¹ßÇöÇü Shuttle Vecto..
pAUR224 DNA
Aspergillus ¿ë Shuttle Vector
pAUR316 DNA
Chromosomal Integrating Çü Shuttle Vector
pPTR I DNA
ÀÚÀ² º¹Á¦Çü Shuttle Vector
pPTR II DNA