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Cloning °ü·Ã
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Genomic DNA ÁõÆø¿ë Kit
> GenomeWalkerKit
GenomeWalkerKit
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Clontech
636405
Universal GenomeWalker 2.0
3 ȸ ±¸Ãà & 80 walks
(Package)
1,546,400¿ø
1,933,000¿ø
11.01 ~ 12.27
,
,
Clontech
639201
Advantage 2 Polymerase Mix
100 ȸ
470,400¿ø
588,000¿ø
11.01 ~ 12.27
,
Clontech
639202
Advantage 2 Polymerase Mix
500 ȸ
1,876,000¿ø
2,345,000¿ø
11.01 ~ 12.27
Clontech
639206
Advantage 2 PCR Kit
100 ȸ
812,000¿ø
1,015,000¿ø
11.01 ~ 12.27
,
Clontech
639207
Advantage 2 PCR Kit
30 ȸ
264,000¿ø
330,000¿ø
11.01 ~ 12.27
Product index
½Ä¹°¿ë Vectors
Takara Plant ½ÇÇè °ü·Ã..
Agrobacterium tumefaci..
pRI 101 DNA ½Ã¸®Áî
pRI 201 DNA ½Ã¸®Áî
pRI 909/910 DNA
È¿¸ð ¡¤ °è»ó±Õ ¿ë Vectors
pAUR101 DNA
pAUR112 DNA
pAUR123 DNA
pAUR135 DNA
pAUR224 DNA
pAUR316 DNA
pPTR I DNA
pPTR II DNA
Genomic DNA
Genomic DNA
Thermus thermophilus H..
¥ëDNA
¥õX174 RF I DNA
DNA Ligation
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Blunting Kit
DNA Ligation Kit
DNA Ligation Kit £¼Mig..
TaKaRa DNA Ligation Ki..
TA-Cloning, ÆòÈ°¸»´Ü Cloni..
½ÇÇè ¸ñÀûº°·Î ÃßõÇÏ´Â..
DNA Fragmentation Kit
Kilo-Sequence ¿ë Delet..
Mighty Cloning Reagent..
Mighty TA-cloning Kit
Mighty TA-cloning Reag..
TaKaRa LA PCR¢â in vit..
TaKaRa T-Vector pMD20
In-Fusion Cloning
[Overview] In-Fusion¢ç..
[°³¿ä] So Easy Product
Online Tools for In-Fu..
[NEW] In-Fusion¢ç Snap..
[NEW] µ¿°á°ÇÁ¶ In-Fusi..
[Kit] In-Fusion¢ç Clon..
In-Fusion¢ç Ready Vect..
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit
Vector (Cloning ¿ë)
M13 mp18 Single Strand..
pBR322 DNA
pKF18k-2/19k-3 DNA
pSTV28/29 DNA
pUC118/119 type vector
pUC18/19 type vector
TaKaRa T-Vector pMD20
Competent Cells & Electro-..
Agrobacterium tumefaci..
Chaperone Competent Ce..
E. coli Competent Cell..
E. coli Competent Cell..
E. coli Electro-Cells ..
¼ö½ÄÈ¿¼Ò¿¡ ´ëÇÏ¿©
¼ö½ÄÈ¿¼Ò ¼øµµ À϶÷
¼ö½ÄÈ¿¼Òº° ¿ëµµ
¼ö½ÄÈ¿¼Ò£¯Ligase
E. coli DNA Ligase
T4 DNA Ligase
T4 RNA Ligase
¼ö½ÄÈ¿¼Ò£¯Alkaline Phospha..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
Alkaline Phosphatase (..
T4 Polynucleotide Kina..
¼ö½ÄÈ¿¼Ò£¯DNA Polymerase¡¢..
DNA Polymerase I (E. ..
Klenow Fragment (Large..
PrimeCap¢â T7 RNA Poly..
T4 DNA Polymerase
Terminal Deoxynucleoti..
¼ö½ÄÈ¿¼Ò£¯Nuclease¡¢Topois..
BAL 31 Nuclease
DNA Topoisomerase I
Exonuclease I
Exonuclease III
Micrococcal Nuclease
Mung Bean Nuclease
NucleoGone¢â Endonucle..
Recombinant DNase I (R..
Ribonuclease H (RNase ..
S1 Nuclease
Nucleotide
Deoxyribonucleoside 8..
dNTP Mixture
¹èÁö ¡¤ Buffer (ºÐ¸»)
AccuGENE 20¡¿SSPE Buff..
Bovine Serum Albumin S..
IPTG£¨dioxane free£©£¨..
X-Gal£¨5-Bromo-4-Chlor..
±× ¿Ü (À¯Àü°øÇÐ °ü·Ã)
DNA-OFF¢ç
Proteinase K
Westase (È¿¸ð ¼¼Æ÷º® ..
Yatalase
Yatalase -Plus-
ºü¸¥ genomic DNAÀÇ PCR-based walking
Exon-intron junctions, promoters, ¶Ç´Â ´Ù¸¥ regulatory element¸¦ Ž»öÇϴµ¥ ÀûÇÕ
¹ÌÁöÀÇ ¼¿À» ½Å¼ÓÇÏ°Ô È®ÀÎ
GenomeWalker Kit´Â expressed sequence tag (EST)°ú °°ÀÌ ÀÌ¹Ì ¾Ë°í ÀÖ´Â genomic DNA ¼¿·ÎºÎÅÍ PCRÀ» ÀÌ¿ëÇÏ¿© ¹ÌÁöÀÇ ¼¿À» È®ÀÎÇϱâ À§ÇÑ Á¦Ç°ÀÌ´Ù. GenomeWalker´Â ±ä ¼¿À» ÁõÆøÇÏ´Â PCR ±â¹ý (long-distance PCR)À» ÀÌ¿ëÇÏ¿© °¢ ´Ü°è¸¶´Ù genomic DNA 6 kb ÀÌ»óÀ» È®ÀÎÇÒ ¼ö ÀÖ´Ù. WalkingÀº ÀÌÀü ´Ü°è¿¡¼ ¾òÀº ¿°±â¼¿À» ±âº»À¸·Î »õ·Î¿î primer¸¦ Á¦ÀÛÇÏ¿© WalkingÀ» ¹Ýº¹ÇÏ´Â °Í¸¸À¸·Î °£´ÜÇÏ°Ô ¹üÀ§¸¦ ³ÐÈú ¼ö ÀÖ´Ù.
GenomeWalker Universal Kit
GenomeWalker Universal Kit´Â Á¾¿¡ »ó°ü¾øÀÌ ±× Á¾ÀÇ genomeÀ¸·ÎºÎÅÍ GenomeWalker library¸¦ Á¦ÀÛÇÒ ¼ö ÀÖ´Ù. ÀÌ Universal Kit´Â °¢±â ´Ù¸¥ 3Á¾ÀÇ ½Ã·á·ÎºÎÅÍ library¸¦ Á¦ÀÛÇÏ¿© 80ȸÀÇ walk¸¦ ÁøÇà ÇÒ ¼ö ÀÖ´Ù.
Kit¸¦ ÀÌ¿ëÇÒ ¶§´Â °¢°¢ÀÇ À¯ÀüÀÚ¿¡ ¸Â°Ô gene-specific primer (GSP)¸¦ Á¦ÀÛÇØ¾ß Çϸç, long-distanc PCR (LD-PCR)¿¡ ÀûÇÕÇÑ DNA polymerase¸¦ º°µµ·Î ±¸¸ÅÇØ¾ß ÇÑ´Ù. Clontech¿¡¼´Â Advantage 2 Polymerase Mix ¶Ç´Â Advantage 2 PCR Kit¸¦ ÃßõÇÑ´Ù.
Universal GenomeWalker 2.0
Universal GenomeWalker 2.0Àº Á¾¿¡ »ó°ü¾øÀÌ ±× Á¾ÀÇ genomic DNAÀ¸·ÎºÎÅÍ GenomeWalker library¸¦ Á¦ÀÛ¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇԵǾî ÀÖ´Ù.
ÀÌ Å°Æ®¿¡´Â library¸¦ ±¸ÃàÇÒ ¼ö ÀÖ´Â component (PCR polymerase Æ÷ÇÔ), genomic DNA Á¤Á¦ kit, digested DNA clean-up kit°¡ Æ÷ÇԵǾî ÀÖ´Ù.
ÀÌ Universal Kit´Â °¢±â ´Ù¸¥ 3Á¾ÀÇ ½Ã·á·ÎºÎÅÍ library¸¦ Á¦ÀÛÇÏ¿© 80ȸÀÇ walk¸¦ ÁøÇà ÇÒ ¼ö ÀÖ´Ù.
Kit¸¦ ÀÌ¿ëÇÒ ¶§´Â °¢°¢ÀÇ À¯ÀüÀÚ¿¡ ¸Â°Ô gene-specific primer (GSP)¸¦ Á¦ÀÛÇØ¾ß ÇÑ´Ù.
Figure 1. The GenomeWalker method.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Keyword :
Genomic DNA ÁõÆø¿ë Kit
GenomeWalkerKit