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Home > ÀüÁ¦Ç°º¸±â > Cloning °ü·Ã > In-Fusion Cloning > [NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly
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[NEW] µ¿°á°ÇÁ¶ In-Fusion¢ç Snap Assembly

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Clontech
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In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix
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8 ȸ
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Clontech
638955
In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix
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779,200¿ø 
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In-Fusion¢ç Snap Assembly EcoDry¢â Master Mix (96 well plate)
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96 ȸ
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Clontech
636763
Stellar¢â Competent Cells
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10 ȸ (10 x 100ul)
234,400¿ø 
293,000¿ø
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Clontech
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Stellar¢â Competent Cells
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¡ØÂü°í»çÇ×
Ÿ»ç ´ëºñ ¶Ù¾î³­ Á¤È®µµ¸¦ °¡Áø In-Fusion¢çÀÇ ÀÚ¼¼ÇÑ Â÷ÀÌÁ¡Àº ´ÙÀ½ ÀڷḦ Âü°íÇϼ¼¿ä!
>>Gibson¡¯s method¿Í ¿ø¸®ºÎÅÍ °á°ú±îÁö ´Ù¸¥ In-Fusion¢ç Snap


  • »ç¿ë Æí¸®¼º - 1ȸ »ç¿ë·®ÀÌ µ¿°á°ÇÁ¶ »óÅ·ΠÁ¦°øµÇ¾î ½Ç¿Â¿¡¼­ º¸°ü °¡´ÉÇÏ°í, handling ¿À·ù³ª ¿À¿° °¡´É¼ºÀÌ ³·¾Æ ½ÇÇè¾çÀÌ ¸¹À» ¶§ ºü¸£°Ô °á°ú¸¦ ¾òÀ» ¼ö ÀÖÀ½.
  • Subcloning ºÒÇÊ¿ä - Cloning À§Ä¡, insert ¼­¿­¿¡ °ü°è¾øÀÌ, ¸ñÀû vector¿¡ ¹Ù·Î cloning
  • ³ôÀº È¿À² - 0.5~15 kbÀÇ ´Ù¾çÇÑ Å©±âÀÇ fragment¿¡¼­ 95% ÀÌ»óÀÇ Á¤È®µµ
  • Seamless construction - ºÒÇÊ¿äÇÑ ¿°±â Ãß°¡ ¾øÀÌ cloning ¿Ï·á (e.g. Á¦ÇÑÈ¿¼Ò »ç¿ë ½Ã, TA cloning ½Ã)
  • ´Ù¾çÇÑ application - Single insert cloningÀº ¹°·Ð multiple cloning, mutagenesis µî Àû¿ë °¡´É (´õ ¸¹Àº Àû¿ë »ç·Ê¸¦ º¸½Ã·Á¸é Ŭ¸¯Çϼ¼¿ä)
Á¦Ç°¼³¸í
In-Fusion¢ç Snap Assembly EcoDry¢âÀº cloning ½Ã¾àÀÌ tube³ª well-plate ³»¿¡ 1ȸ¾¿ ºÐÁÖ, µ¿°á°ÇÁ¶µÈ ÇüÅ·Î, ½Ç¿Â¿¡¼­ º¸°ü °¡´ÉÇϱ⠶§¹®¿¡ liquid handler¿Í °°Àº ÀÚµ¿È­ ±â±â¿¡¼­ high-throughput cloningÀ» ¸Å¿ì Æí¸®ÇÏ°Ô ÁøÇàÇÒ ¼ö ÀÖ´Ù. Á÷Á¢ ½ÇÇèÀ» Çϰųª ÀÚµ¿È­ Àåºñ¸¦ ÀÌ¿ëÇÒ ¶§ ¸ðµÎ¿¡¼­ handlingÀ» ÃÖ¼ÒÈ­ ÇÒ ¼ö ÀÖ¾î ÀÌ·Î ÀÎÇÑ error³ª ¿À¿°À» ¹æÁöÇÒ ¼ö ÀÖ´Ù. »Ó¸¸ ¾Æ´Ï¶ó Liquid typeÀÇ ½Ã¾à°ú ¸¶Âù°¡Áö·Î PCR »ê¹°ÀÇ Á¦ÇÑÈ¿¼Ò 󸮳ª ligation, blunting ¾øÀ̵µ cloningÀ» single stepÀ¸·Î ¿Ï·áÇÒ ¼ö ÀÖ´Ù.

In-Fusion¢ç ±â¼ú Ư¼º »ó ¾î¶² vectorµç Á¦ÇÑÈ¿¼Ò ÀÚ¸®¿¡ »ó°ü¾øÀÌ, subcloningÀ̳ª º°µµÀÇ Á¶ÀÛ ¾øÀÌ insert¸¦ »ðÀÔÇÒ ¼ö ÀÖÀ¸¸ç, cloning °úÁ¤ÀÌ ¸Å¿ì °£´ÜÇϱ⿡ high-throughputÀ¸·Î ½ÇÇèÀÌ °¡´ÉÇÏ´Ù. Cloning °úÁ¤¿¡¼­´Â ¼±ÇüÈ­ÇÑ vector¿Í vectorÀÇ ¾ç ¸»´Ü 15 bp¸¦ ºÎ°¡ÇÑ insert¸¦ D.W¿Í ÇÔ²² In-Fusion¢ç Snap Assembly EcoDry¢â¿¡ ÷°¡ÇÏ°Ô µÇ´Âµ¥, ¿Â¶óÀο¡¼­ Á¦°øÇÏ´Â In-Fusion¢ç primer design toolÀ» ÀÌ¿ëÇϸé, °¢°¢ÀÇ ½ÇÇè¿¡ ¾Ë¸ÂÀº primer¸¦ Æí¸®ÇÏ°Ô µðÀÚÀÎÇÒ ¼ö ÀÖ´Ù.

¹ÝÀÀÀÌ ½ÃÀ۵Ǹé, ¼±ÇüÈ­ µÈ DNA °¡´ÚÀÇ 3¡¯ end·ÎºÎÅÍ ¿°±â¼­¿­À» Á¦°ÅÇÏ°í, µÎ °³ÀÇ DNA ´ÜÆí¿¡¼­ »óº¸¼­¿­ °£ÀÇ annealingÀ» ÅëÇØ DNA°¡ °áÇÕ½ÃŲ´Ù. ÀÌ ¹æ¹ýÀº SubcloningÀ̳ª º°µµÀÇ Á¶ÀÛ ¾øÀÌ, ÇϳªÀÇ fragment»Ó ¾Æ´Ï¶ó ¿©·¯ °³ÀÇ fragment¸¦ ÇϳªÀÇ vector¿¡ ¹Ù·Î Àû¿ëÇÒ ¼ö ÀÖ¾î high-throughput ½ÇÇè¿¡ cloningÇÒ ¼ö ÀÖÀ» »Ó¸¸ ¾Æ´Ï¶ó, adapter, linker, protein tag¸¦ »ðÀÔÇϰųª mutagenesis, gene synthesis µîÀÇ application¿¡µµ È°¿ëÇÒ ¼ö ÀÖ´Ù.
Applications
- PCR cloning
- High-throughput cloning (see full range of reagent options on our high-throughput cloning page)
- Multiple-fragment cloning
- Gene synthesis
- Gene design
- Mutagenesis
- Domain swapping
- Domain modification
ÀÚ¼¼ÇÑ ±¸¼ºÀº CoA¸¦ Âü°íÇϼ¼¿ä.

Keyword : infusion,in-fusion,cloning,PCR cloning,cloning,Ŭ·Î´×,º¹¼ö´ÜÆí,ÀÎÇ»Àü,ÇÁ·Î¹Í½º,premix, mutagenesis,mutation,soeasy,so easy,Ecodry,µ¿°á°ÇÁ¶,lyophilized,pre-aliquoted

In-Fusion Cloning
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ÃÖ°íÀÇ Colony Çü¼º´É°ú Á¤È®ÇÑ PCR cloning
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