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qPCR¿ë cDNAÇÕ¼º Premix ½Ã¾à

5x RT Master Mix (qPCR¿ë)

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Takara
RR036A
PrimeScript¢â RT Master Mix (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱ⠻ùÇýÅû 
200 ȸ
(10 §¡ ¹ÝÀÀ°è)
369,000¿ø  RR036A_e.v2202Da.pdf
Takara
RR036B
PrimeScript¢â RT Master Mix (Perfect Real Time)
°ü·ÃÇмú ±¸¸ÅÇϱâ
800 ȸ (RR036A x 4)
1,328,000¿ø 



  • ¿ªÀü»ç ¹ÝÀÀ¿¡ ÇÊ¿äÇÑ ¸ðµç ½Ã¾àÀÌ Æ÷ÇÔµÈ ¿ÏÀü premix ŸÀÔÀ¸·Î »ç¿ëÀÌ °£Æí
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PrimeScript¢â RT Master Mix (Perfect Real Time)Àº 2 step Real Time RT-PCR ¿¡ ÃÖÀûÈ­µÈ ¿ªÀü»ç ¹ÝÀÀ¿ë premix ½Ã¾àÀÌ´Ù.
Real Time RT-PCRÀÇ ¿ªÀü»ç ¹ÝÀÀ¿¡ ÇÊ¿äÇÑ ½Ã¾à (PrimeScript¢â RTase, RNase Inhibitor, Random 6 mers, Oligo dT Primer, dNTP Mixture, reaction buffer)ÀÌ ¸ðµÎ Æ÷ÇÔµÈ 5¡¿premix ½Ã¾àÀ̸ç, ÁÖÇü RNA¿Í H2O¸¦ ÷°¡ÇÏ´Â °Í¸¸À¸·Î ½Å¼ÓÈ÷ ¹ÝÀÀÀ» ¿ªÀü»ç¹ÝÀÀÀ» ÁøÇàÇÒ ¼ö ÀÖ´Ù.
½ÅÀ强ÀÌ ¶Ù¾î³­ PrimeScript¢ç RTase¸¦ »ç¿ëÇÏ¿©, PrimeScript¢â RT reagent Kit (Perfect Real Time) (Code RR037A/B)¿Í °°ÀÌ Real Time PCR¿ë ÁÖÇü cDNA¸¦ ªÀº ½Ã°£¾È¿¡ ³ôÀº È¿À²·Î ÇÕ¼ºÇÒ ¼ö ÀÖ´Ù.
º» Á¦Ç°¿¡ ÀÇÇØ ÇÕ¼ºµÈ cDNA´Â TB Green¢â assay, Dual-Labeled Probe assay¿¡ ¸ðµÎ »ç¿ëÇÒ ¼ö ÀÖÀ¸¸ç, ¸ñÀû¿¡ µû¶ó TB Green¢â Premix Ex Taq¢â II (Tli RNaseH Plus) (Code RR820A), TB Green¢â Fast qPCR Mix (Code RR430A), Probe qPCR Mix (Code RR391A) µî Real Time PCR ½Ã¾à°ú ÇÔ²² »ç¿ëÇÑ´Ù.
³»¿ë (200 ȸ, 10 §¡ ¹ÝÀÀ½Ã)

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5¡¿PrimeScript RT Master Mix (Perfect Real Time) *1

400 §¡

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RNase Free dH2O

1 §¢ x 2

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EASY Dilution (for Real Time PCR) *2

1 §¢


*1 PrimeScript¢ç RTase, RNase Inhibitor, Oligo dT Primer, Random 6 mers, dNTP Mixture ¹× ¹ÝÀÀ ¹öÆÛ (Mg2+ÇÔÀ¯)¸¦ Æ÷ÇÔÇÑ´Ù.
*2 total RNA³ª cDNA¸¦ ´Ü°è Èñ¼®ÇÒ ¶§ Èñ¼®¿ë¾×À¸·Î »ç¿ëÇÑ´Ù. H2O³ª TE buffer·Î Èñ¼®Çϸé Á¤È®ÇÑ Èñ¼®À» ÇÒ ¼ö ¾ø´Â °æ¿ì°¡ ÀÖÁö¸¸, EASY DilutionÀ» ÀÌ¿ëÇϸé Àú³óµµ±îÁö Á¤È®È÷ Èñ¼®À» ÇÒ ¼ö ÀÖ´Ù. º» buffer´Â ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCRÀÇ ¹ÝÀÀ¼º¿¡ ¿µÇâÀ» ¹ÌÄ¡Áö ¾ÊÀ¸¹Ç·Î Èñ¼®ÇÑ ÁÖÇü ¿ë¾×À» ±×´ë·Î ¿ªÀü»ç ¹ÝÀÀÀ̳ª PCR ¹ÝÀÀÀÇ ÁÖÇüÀ¸·Î¼­ »ç¿ëÇÒ ¼ö ÀÖ´Ù.
EASY Dilution (for Real Time PCR) (Code 9160)´Â º°µµ ±¸¸Å °¡´É.

¡¼ÁÖÀÇ¡½ EASY DilutionÀº TakaraÀÇ Real Time PCR½Ã¾à°ú ÇÔ²² »ç¿ëÇϱ⸦ ±ÇÀåÇϸç, Ÿ»ç Á¦Ç°°úÀÇ È£È¯¼ºÀº È®ÀεÇÁö ¾Ê¾ÒÀ½.
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Keyword : fast qRT PCR,ºü¸¥ qPCR,Real Time PCR,qPCR,sybr green,RT kit,cDNAÇÕ¼º

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