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Gene transfer (virus)
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Mammalian Cell ¿ë Vector
> pBApo-EF1¥á Vector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-EF1¥á Vector ½Ã¸®Áî
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Takara
3243
pBApo-EF1¥á Neo DNA
20 ¥ìg
423,000¿ø
Takara
3244
pBApo-EF1¥á Pur DNA
20 ¥ìg
423,000¿ø
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
RetroNectin¢ç Pro (Rec..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
Á¦Ç°¼³¸í
pBApo-EF1¥á´Â Æ÷À¯·ù ¼¼Æ÷¿ë À¯ÀüÀÚ expression vector·Î Human elongation factor-1 ¥á À¯ÀüÀÚ (EF1 ¥á promoter) ¹× Herpes Simple Virus Thymidine KinaseÀÇ polyA signalÀ» žÀçÇÏ°í ÀÖ´Ù. Cloning site¿¡ ¸ñÀû À¯ÀüÀÚÀÇORF¸¦ »ðÀÔÇÏ¿© ¸ñÀû À¯ÀüÀÚÀÇ expression plasmid¸¦ ¾òÀ» ¼ö ÀÖÀ¸¸ç, À¯ÀüÀÚ ¹ßÇö ÀÌ¿Ü¿¡ pri-microRNA µîÀÇ Àü»ç »ê¹° ¹ßÇö¿¡µµ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
¶ÇÇÑ ½±°Ô ¡¸promoter£«ORF£«polyA signal¡¹ cassette¸¦ adenovirus vector·Î ¿Å±æ ¼ö ÀÖÀ¸¸ç, À̶§ ÀçÁ¶ÇÕ adenovirus Á¦ÀÛ¿¡´Â Adenovirus Expression Vector Kit (Dual Version) Ver. 2 (Code 6170)¸¦ ÀÌ¿ëÇÒ ¼ö ÀÖ´Ù.
Adenovirus´Â ³ôÀº °¨¿°È¿À²°ú ³ÐÀº °¨¿°´É·ÂÀ» °¡Áö°í ÀÖ¾î
in vitro
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in vivo
À¯ÀüÀÚ µµÀÔ¿¡ ÀûÀýÇÏ´Ù. pBApo-EF1¥á½Ã¸®Áî¿¡¼´Â mammalian cellÀÇ selection marker·Î neomycin ³»¼º À¯ÀüÀÚ ¶Ç´Â puromycin ³»¼º À¯ÀüÀÚ¸¦ žÀçÇÑ vector°¡ ÀÖ¾î, ½ÇÇè ¸ñÀû¿¡ µû¶ó ÀûÀýÇÑ vector¸¦ ¼±ÅÃÇÒ ¼ö ÀÖ´Ù.
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pBApo-EF1¥á Neo DNA (500 ng/¥ìl)
20 ¥ìg
¶Ç´Â pBApo-EF1¥á Pur DNA (500 ng/¥ìl)
º¸Á¸ -20¡É
Çü»ó
10 mM
Tris - HCl, pH8.0
1 mM
EDTA
pBApo-EF1¥á Neo DNA, pBApo-EF1¥á Pur DNAÀÇ ±¸Á¶
pBApo - EF1¥á Neo , pBApo - EF1¥á Pur ÀÇ Å¬·Î´× ±×¸²
Keyword :
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾·ùÀÇ tag¸¦ ÀÌ¿ëÇÑ ´Ü¹éÁú »ý»ê ¹× Á¤Á¦
Bidirectional Æ÷À¯·ç ¼¼Æ÷ ¹ßÇö Vectors
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bidirectional Expression Vectors)
EF1¥á Promotor Vector (Çü±¤ ´Ü¹éÁú, IRES ±âŸ)
epitope tag (HA or c-Myc) Vectors
in vivo Vector, pLIVE Vector ½Ã¸®Áî
Magnetic Myc IP Kit
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-CMVvector ½Ã¸®Áî
Æ÷À¯µ¿¹° ¼¼Æ÷ ¹ßÇö Vector
pBApo-EF1¥á Vector ½Ã¸®Áî
pIRES Bicistronic Expression Vector