AAV 추출 시약

AAV Extraction Solution A

2 x 1.5 ㎖

AAV Extraction Solution B

300 ㎕

2.

AAV genome 간이 추출 (100 회)

DNase I

100 ㎕

10×DNase I Buffer

200 ㎕

Lysis Buffer

2 x 1 ㎖

3.

Real Time PCR (25 ㎕ 반응 시, 100회)

TB Green™ Premix Ex Taq II (Tli RNaseH Plus)

2 x 625 ㎕

AAV Forward Titer Primer

20 ㎕

AAV Reverse Titer Primer

20 ㎕

dH₂O

3 x 1 ㎖

ROX Reference Dye

50 ㎕

ROX Reference Dye II

50 ㎕

Positive Control

50 ㎕

EASY Dilution (for Real Time PCR)

5 x 1 ㎖