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Adeno-X GoStix

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Clontech
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Adeno-X GoStix
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20 tests
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11.01 ~ 12.27
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Adeno-X GoStix´Â ¼¼Æ÷º¯¼ºÀÇ ¿µÇâÀÌ °üÂûµÇ±â Àü ¾Æµ¥³ë¹ÙÀÌ·¯½ºÀÇ »óŸ¦ ½Å¼ÓÇÏ°í °£ÆíÇÏ°Ô Å×½ºÆ®ÇÒ ¼ö ÀÖ´Â Á¦Ç°À¸·Î ½Ã°£°ú ³ë·ÂÀ» Àý¾àÇÒ ¼ö ÀÖ´Ù. ¼¼Æ÷¹è¾ç »óÃþ¾× 20 ¥ìl¸¦ GoStixÀÇ cassette¿¡ ³Ö°í buffer 4¹æ¿ï¸¸ ¶³¾îÆ®¸° ÈÄ 2~20ºÐ Á¤µµ¸¸ ±â´Ù¸®¸é ¹êµå°¡ ³ªÅ¸³­´Ù. ÀÌ Á¦Ç°À» ÀÌ¿ëÇÏ¸é ¾Æµ¥³ë¹ÙÀÌ·¯½ºÀÇ ³óÃà °úÁ¤¿¡¼­ °£´ÜÇÑ Å×½ºÆ®¸¸À¸·Îµµ ¹ÙÀÌ·¯½º ¿ª°¡°¡ ÃæºÐÇÑÁö È®ÀÎ ÇÒ ¼ö Àֱ⠶§¹®¿¡ ³óÃàÀÇ ÀûÁ¤ ½ÃÁ¡À» °áÁ¤ÇÒ ¼ö ÀÖ´Ù. ¹êµå°¡ ÁøÇÒ¼ö·Ï ¾Æµ¥³ë¹ÙÀÌ·¯½ºÀÇ ¿ª°¡°¡ ³ôÀ½À» ÀǹÌÇÑ´Ù.



Rapid adenovirus detection during amplification. Panel A. Adenovirus expressing ZsGreen1 was amplified in HEK293 cells. 48 hr post-infection, and each day thereafter, the supernatant and the cell pellet were harvested and titers were quantified by counting fluorescent cells in an infectious unit (IFU) assay. The increase in virus level in the cell pellets was mirrored by a proportional increase in the amount of virus collected from the culture supernatant. The amount of virus in the supernatant remained < 10% of the total virus output. Panel B. Supernatant serves as clear indicator of total yields. In addition to IFU determination, 20 ¥ìl of each sample was applied to an Adeno-X GoStix. The increase in band intensity paralleled the increase in virus production observed by infectious unit titer.



Use Adeno-X GoStix to instantly confirm the presence of adenovirus in cell culture supernatants or lysates. The test, which detects adenoviral hexon proteins, requires just 20 ¥ìl of adenoviral supernatant to determine if infectious virus was produced posttransfection, or to select the best time to harvest your virus following amplification. The included Hexon Control (bottom) confirms GoStix test function.

Keyword : ¿ª°¡ÃøÁ¤,Titration,Titer,ŸÀÌÅÍ,¿ª°¡,ELISA,¹ÙÀÌ·¯½º¿ª°¡,¹ÙÀÌ·¯½º ¿ª°¡,Virus genome copies,Virus copy,Virus copies,Adenovirus ¿ª°¡

Adenovirus
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Real Time PCR·Î Genome copy ¼ö¸¦ ½Å¼ÓÇÏ°Ô Ãø..
qPCR titer ÃøÁ¤ Kit
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Hexon ±â¹Ý titer ÃøÁ¤
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Adeno-X GoStix