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Home > ÀüÁ¦Ç°º¸±â > Gene transfer (virus) > Retrovirus > MSCV Retrovirus ½Ã½ºÅÛ

MSCV Retrovirus ½Ã½ºÅÛ

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Á¦Á¶»ç Á¦Ç°ÄÚµå Á¦Ç°¸í ¿ë·® °¡°Ý
(ºÎ°¡¼¼º°µµ)
ºñ°í »ç¿ëÀڸŴº¾ó
Clontech
634401
MSCV Retroviral Expression System
°ü·ÃÇмú ±¸¸ÅÇϱâ LMOÁ¦Ç°  ¶óÀ̼±½º 
1 Set
(Package)
1,380,000¿ø 
1,725,000¿ø
°¡°ÝÇÒÀÎ
11.01 ~ 12.27
Á¦Á¶»ç ÆäÀÌÁö·Î ¹Ù·Î°¡±â
x33005, x33223


* º» Á¦Ç°±ºÀ» ¿µ¸® ¸ñÀûÀÇ ¿¬±¸½Ã¼³ ¶Ç´Â ¹ýÀÎ °í°´ÀÌ ±¸¸ÅÇÏ´Â °æ¿ì, »ç¿ë¸ñÀû¿¡ »ó°ü¾øÀÌ Á¦Ç° ±¸ÀÔ¿¡ ¾Õ¼­ Takara Bio USA·ÎºÎÅÍ À¯»ó ¶óÀ̼±½º Ãëµæ°ú ¶óÀ̼±½º È®Àμ­°¡ ÇÊ¿äÇÕ´Ï´Ù. ¶óÀ̼±½º ÃëµæÀ» ¿øÇϽô °æ¿ì, ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î º°µµ ¹®ÀÇ ¹Ù¶ø´Ï´Ù.

  • Á¶Ç÷¼¼Æ÷ (hematopoietic cell), ¹è¾ÆÁٱ⼼Æ÷ (ES cell) ±×¸®°í ¹è¾Æ¾Ï¼¼Æ÷ (EC cell)¿¡¼­ÀÇ È¿°úÀûÀÎ À¯ÀüÀÚ¹ßÇö
  • 3 Á¾ÀÇ ¼±º° ¸¶Ä¿¸¦ ¼±Åà °¡´É
  • PT67 packaging cell lineÀ» Æ÷ÇÔÇÑ ¿Ïº®ÇÑ retroiviral system
MSCV (Murine Stem Cell Virus) Retroviral Expression SystemÀº murine ¶Ç´Â Àΰ£ Á¶Ç÷¼¼Æ÷ (hematopoietic cell), ¹è¾ÆÁٱ⼼Æ÷(embryonic cell line, ES), ¹è¾Æ¾Ï¼¼Æ÷ (embryonal carcinoma cell, EC cell)¸¦ Æ÷ÇÔÇÑ pluripotent cell line¿¡¼­ ¸ñÀûÀ¯ÀüÀÚ¸¦ µµÀÔÇÏ°í ¹ßÇö½ÃÅ°±â À§ÇÏ¿© ÃÖÀûÈ­µÈ vectorµé·Î ±¸¼ºµÇ¾î ÀÖ´Ù. ÀÌµé ½Ã½ºÅÛÀº Æ÷À¯·ù ¼¼Æ÷¿¡µµ È¿°úÀûÀ¸·Î Àû¿ëµÉ ¼ö ÀÖÀ¸¸ç (1-3), ¼¼Æ÷¹è¾ç°ú À¯ÀüÀÚ µµÀÔ ºÐ¼®¿¡ À־ ºÐÈ­ (development), ¹è¹ß»ý (embryogenesis) ¶Ç´Â ¸é¿ª¹ÝÀÀ (immune response) °úÁ¤¿¡ °ü¿©ÇÏ´Â À¯ÀüÀÚÀÇ ±â´É ºÐ¼®¿¡ ÀÌ»óÀûÀÌ´Ù.
InfectionÀÌ ¾î·Á¿î ¼¼Æ÷¸¦ À§ÇÑ ¼³°è
MSCV SystemÀº pMSCVneo, pMSCVhyg ±×¸®°í pMSCVpuroÀÇ 3 °¡Áö vector¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. ÀÌ vectorµéÀº µµÀÔÀÌ ¾î·Á¿î cell line¿¡ Àû¿ëÇÒ ¼ö ÀÖµµ·Ï murine stem cell PCMV virus·ÎºÎÅÍ Æ¯º°È÷ Á¦ÀÛµÈ long terminal repeat (LTR)À» °¡Áö°í ÀÖ´Ù. ÀÌ LTRÀº ES¼¼Æ÷¿Í EC¼¼Æ÷¿¡¼­ Àü»çÈ°¼ºÈ­¸¦ ÁõÁø½ÃÅ´°ú µ¿½Ã¿¡ Àü»ç¾ïÁ¦ÀÛ¿ëÀ» ¹æÁöÇÒ ¼ö ÀÖµµ·Ï ¿©·¯ °³ÀÇ point mutation°ú ÇϳªÀÇ deletion¸¦ °¡Áö°í ÀÖ´Ù´Â Á¡¿¡¼­ MMLV LTR°ú´Â ´Ù¸£´Ù. °á°úÀûÀ¸·Î, PCMV LTRÀº Áٱ⼼Æ÷³ª ´Ù¸¥ Æ÷À¯·ù cell line¿¡¼­ ¸ñÀû À¯ÀüÀÚ°¡ ±¸Á¶ÀûÀ¸·Î °í¹ßÇö µÇµµ·Ï ÇÑ´Ù. MSCV ½Ã½ºÅÛÀº RetroPack PT67 Packaging Cell Line¸¦ Æ÷ÇÔÇÏ°í ÀÖÀ¸¸ç, ÀÌ packaging cell lines´Â ±¤¹üÀ§ÇÑ Æ÷À¯·ù ¼÷ÁÖ ¼¼Æ÷¸¦ ÃæºÐÈ÷ °¨¿°½Ãų ¼ö ÀÖ´Â °í¿ª°¡ÀÇ ¹ÙÀÌ·¯½º¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.


Figure 1. pMSCV Vectors map.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)

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