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Gene transfer (virus)
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Retrovirus
> MSCV Retrovirus ½Ã½ºÅÛ
MSCV Retrovirus ½Ã½ºÅÛ
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Clontech
634401
MSCV Retroviral Expression System
1 Set
(Package)
1,380,000¿ø
1,725,000¿ø
11.01 ~ 12.27
,
Product index
Adeno-Associated Virus (AA..
Overview - All about A..
Packaging (293T Cell L..
AAV1, 2, 5, 6 Vector S..
CRE Recombinase System
LacZ System
pAAV-ZsGreen1 Vector
CRISPR/Cas9 Vector Sys..
CRISPR/SaCas9 Vector S..
Tet-Inducible System (..
Cell & Sup. Purificati..
Purification Kit Maxi/..
Purification Kit (AAV2..
AAVpro¢ç Concentrator
AAVpro¢ç Titration Kit..
Extraction Solution
AAV Freeze-thaw extrac..
Lentivirus
[°³¿ä] Packaging Singl..
[FAQ] Lentiviral Gene ..
CMV promoter º¤ÅÍ ½Ã½º..
EF1 ¥á promoter º¤ÅÍ ..
Lenti-X¢â SARS-CoV-2 P..
3¼¼´ë Lentivirus packa..
Çü±¤´Ü¹éÁú ¸®Æ÷ÅÍ º¤ÅÍ
Tet-on inducible Expre..
Tet One Inducible Expr..
Lenti-X¢â CRISPR/Cas9 ..
(Legacy) Tet-Inducible..
Reporter assay ½Ã½ºÅÛ ..
Packaging single shots
Packaging Cell Lines
Lenti-X¢â qRT-PCR Titr..
Lenti-X¢â p24 Rapid Ti..
GoStix¢â Plus
Lentivirus ³óÃà ½Ã¾à
Lentivirus Á¤Á¦
Ecotropic Receptor Boo..
RetroNectin¢ç (Recombi..
Transduction ½Ã¾à
Provirus Quantitation ..
Integration Site Analy..
Lenti-X¢â Transduction..
RetroNectin¢ç Pro (Rec..
TransIT¢ç Lentivirus S..
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ..
Packaging Cell £¦ ½Ã½º..
Retrovirus vector £¦ ..
Çü±¤´Ü¹éÁú Retrovirus ..
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
pDON-5 DNA
pDON-AI-2 DNA
pMEI-5 DNA
Retrovirus Packaging K..
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (T..
Tet One Inducible Syst..
qRT-PCR titer ÃøÁ¤
Titer Set (for Real Ti..
Retrovirus ³óÃà
Receptor Boosters (Eco..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analy..
Adenovirus
Adeno-X¢â workflow
Adenoviral Expression ..
Packaging Cell Lines
Adenovirus Á¤Á¦ (Maxi ..
Adenovirus Á¤Á¦ (Mega ..
qPCR titer ÃøÁ¤ Kit
Hexon ±â¹Ý titer ÃøÁ¤
Adeno-X GoStix
CAR Receptor Booster
Mammalian Cell ¿ë Vector
[¼±Åð¡À̵å] ´Ù¾çÇÑ Á¾..
¾ç¹æÇ⼺ ¹ßÇö º¤ÅÍ (Bi..
EF1¥á Promotor Vector ..
epitope tag (HA or c-M..
in vivo Vector, pLIVE ..
Magnetic Myc IP Kit
pBApo-CMVvector ½Ã¸®Áî
pBApo-EF1¥á Vector ½Ã..
pIRES Bicistronic Expr..
* º» Á¦Ç°±ºÀ» ¿µ¸® ¸ñÀûÀÇ ¿¬±¸½Ã¼³ ¶Ç´Â ¹ýÀÎ °í°´ÀÌ ±¸¸ÅÇÏ´Â °æ¿ì, »ç¿ë¸ñÀû¿¡ »ó°ü¾øÀÌ Á¦Ç° ±¸ÀÔ¿¡ ¾Õ¼ Takara Bio USA·ÎºÎÅÍ
À¯»ó ¶óÀ̼±½º Ãëµæ°ú ¶óÀ̼±½º È®Àμ
°¡ ÇÊ¿äÇÕ´Ï´Ù. ¶óÀ̼±½º ÃëµæÀ» ¿øÇϽô °æ¿ì, ´ÙÄ«¶óÄÚ¸®¾Æ (02-2081-2510)·Î º°µµ ¹®ÀÇ ¹Ù¶ø´Ï´Ù.
Á¶Ç÷¼¼Æ÷ (hematopoietic cell), ¹è¾ÆÁٱ⼼Æ÷ (ES cell) ±×¸®°í ¹è¾Æ¾Ï¼¼Æ÷ (EC cell)¿¡¼ÀÇ È¿°úÀûÀÎ À¯ÀüÀÚ¹ßÇö
3 Á¾ÀÇ ¼±º° ¸¶Ä¿¸¦ ¼±Åà °¡´É
PT67 packaging cell lineÀ» Æ÷ÇÔÇÑ ¿Ïº®ÇÑ retroiviral system
MSCV (Murine Stem Cell Virus) Retroviral Expression SystemÀº murine ¶Ç´Â Àΰ£ Á¶Ç÷¼¼Æ÷ (hematopoietic cell), ¹è¾ÆÁٱ⼼Æ÷(embryonic cell line, ES), ¹è¾Æ¾Ï¼¼Æ÷ (embryonal carcinoma cell, EC cell)¸¦ Æ÷ÇÔÇÑ pluripotent cell line¿¡¼ ¸ñÀûÀ¯ÀüÀÚ¸¦ µµÀÔÇÏ°í ¹ßÇö½ÃÅ°±â À§ÇÏ¿© ÃÖÀûÈµÈ vectorµé·Î ±¸¼ºµÇ¾î ÀÖ´Ù. ÀÌµé ½Ã½ºÅÛÀº Æ÷À¯·ù ¼¼Æ÷¿¡µµ È¿°úÀûÀ¸·Î Àû¿ëµÉ ¼ö ÀÖÀ¸¸ç (1-3), ¼¼Æ÷¹è¾ç°ú À¯ÀüÀÚ µµÀÔ ºÐ¼®¿¡ ÀÖ¾î¼ ºÐÈ (development), ¹è¹ß»ý (embryogenesis) ¶Ç´Â ¸é¿ª¹ÝÀÀ (immune response) °úÁ¤¿¡ °ü¿©ÇÏ´Â À¯ÀüÀÚÀÇ ±â´É ºÐ¼®¿¡ ÀÌ»óÀûÀÌ´Ù.
InfectionÀÌ ¾î·Á¿î ¼¼Æ÷¸¦ À§ÇÑ ¼³°è
MSCV SystemÀº pMSCVneo, pMSCVhyg ±×¸®°í pMSCVpuroÀÇ 3 °¡Áö vector¸¦ Æ÷ÇÔÇÏ°í ÀÖ´Ù. ÀÌ vectorµéÀº µµÀÔÀÌ ¾î·Á¿î cell line¿¡ Àû¿ëÇÒ ¼ö ÀÖµµ·Ï murine stem cell PCMV virus·ÎºÎÅÍ Æ¯º°È÷ Á¦ÀÛµÈ long terminal repeat (LTR)À» °¡Áö°í ÀÖ´Ù. ÀÌ LTRÀº ES¼¼Æ÷¿Í EC¼¼Æ÷¿¡¼ Àü»çÈ°¼ºÈ¸¦ ÁõÁø½ÃÅ´°ú µ¿½Ã¿¡ Àü»ç¾ïÁ¦ÀÛ¿ëÀ» ¹æÁöÇÒ ¼ö ÀÖµµ·Ï ¿©·¯ °³ÀÇ point mutation°ú ÇϳªÀÇ deletion¸¦ °¡Áö°í ÀÖ´Ù´Â Á¡¿¡¼ MMLV LTR°ú´Â ´Ù¸£´Ù. °á°úÀûÀ¸·Î, PCMV LTRÀº Áٱ⼼Æ÷³ª ´Ù¸¥ Æ÷À¯·ù cell line¿¡¼ ¸ñÀû À¯ÀüÀÚ°¡ ±¸Á¶ÀûÀ¸·Î °í¹ßÇö µÇµµ·Ï ÇÑ´Ù. MSCV ½Ã½ºÅÛÀº RetroPack PT67 Packaging Cell Line¸¦ Æ÷ÇÔÇÏ°í ÀÖÀ¸¸ç, ÀÌ packaging cell lines´Â ±¤¹üÀ§ÇÑ Æ÷À¯·ù ¼÷ÁÖ ¼¼Æ÷¸¦ ÃæºÐÈ÷ °¨¿°½Ãų ¼ö ÀÖ´Â °í¿ª°¡ÀÇ ¹ÙÀÌ·¯½º¸¦ »ý»êÇÒ ¼ö ÀÖ´Ù.
Figure 1. pMSCV Vectors map.
Components & Storage Conditions
°¢ Á¦Ç° ±¸¼º¹°°ú º¸°üÁ¶°ÇÀº Certificate of Analysis ¸¦ ÂüÁ¶ÇϽʽÿÀ.
Cell Line Ãë±Þ ÁÖÀÇ»çÇ× (Ŭ¸¯)
Keyword :
Retrovirus
Retro-X ¼±Åà °¡À̵å
RetroNectin¢çÀÇ ´Ù¾çÇÑ ÃֽŠapplication
Packaging Cell £¦ ½Ã½ºÅÛ
Retrovirus vector £¦ ½Ã½ºÅÛ
Çü±¤´Ü¹éÁú Retrovirus vector
MSCV Retrovirus ½Ã½ºÅÛ
Retro-X Q vector
À¯ÀüÀÚ µµÀÔ È¿À²°ú ¹ßÇö ±ÕÇü¼ºÀÌ ÁÁ¾Æ Ãʺ¸ ..
pDON-5 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔ °¡´ÉÇÑ Retrovirus Vecto..
pDON-AI-2 DNA
°íÈ¿À²·Î À¯ÀüÀÚ µµÀÔÀÌ °¡´ÉÇÑ Retrovirus vec..
pMEI-5 DNA
ÀϽÃÀûÀ¸·Î ³ôÀº titerÀÇ Recombinant Retrovir..
Retrovirus Packaging Kit
Tet À¯µµ¹ßÇö ½Ã½ºÅÛ (Tet-inducible-two vectors)
Single vectorÀÇ Retro-viral Tet À¯µµ¹ßÇö ½Ã..
Tet One Inducible System (one vector)
4½Ã°£ À̳»¿¡ Recombinant RetrovirusÀÇ RNA ti..
qRT-PCR titer ÃøÁ¤
Virus »óû¾×¿¡¼ Á÷Á¢ 4 ½Ã°£¿¡ RNA titer ¸¦ ..
Titer Set (for Real Time PCR)
Retrovirus ³óÃà
Receptor Boosters (Ecotropic and Amphotropic)
Cycling Probe·Î ½Å¼ÓÇÏ°Ô Provirus Copy ¼ö¸¦ ..
Provirus Copy ¼ö ÃøÁ¤
Integration Site Analysis Kit